LOCUS       sequence-89994-e        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET StrepII-StrepII TEV LIC cloning vector (2RRT)
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(207..311)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     379..397
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(424..429,430..446,447..452)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(818..865)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     861..879
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
       61 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
      121 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
      181 gcgacacgga aatgttgaat actcatactc ttcctttttc aatgttattg aagcatttat
      241 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
      301 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
      361 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattctca
      421 tgtttgacag cttatcatcg ataagcttta atgcggtagt ttatcacagt taaattgcta
      481 acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt
      541 caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga
      601 tgtcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt
      661 gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc
      721 agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc
      781 cgtcctgtgg atgtccggat atagttcctc ctttcagcaa aaaacccctc aagacccgtt
      841 tagaggcccc aaggggttat gctagttatt gctcagcggt ggcagcagcc aactcagctt
      901 cctttcgggc tttgttagca gccggatcag cgcgcacgcg tggtaccggc cggccaccag
      961 gtggcgcgcc gcgatcgcgg atccttatgg agttgggatt tatccacttc
//
