LOCUS       sequence-89998-e        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET StrepII-StrepII TEV LIC cloning vector (2RRT)
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     201..220
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     304..323
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        304..322
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             411..433
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             453..476
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             492..515
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             516..536
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(675..693)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      689..736
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
       61 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
      121 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
      181 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
      241 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
      301 aattaatacg actcactata gggagaccac aacggtttcc ctctagtgcc ggctccggag
      361 agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat tttgtttaac
      421 tttaagaagg agatatagat atgaaatctt cttggagcca tccgcagttt gaaaaaggcg
      481 ccatgaccgg ttggtcccac ccgcagttcg aaaaagaaaa cctgtacttc caatccaata
      541 ttggaagtgg ataaatccca actccataag gatccgcgat cgcggcgcgc cacctggtgg
      601 ccggccggta ccacgcgtgc gcgctgatcc ggctgctaac aaagcccgaa aggaagctga
      661 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
      721 cttgaggggt tttttgctga aaggaggaac tatatccgga catccacagg acgggtgtgg
      781 tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc
      841 ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat
      901 atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg acatcccgca
      961 agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc
     1021 cgaggatgac gatgagcgca ttgttagatt tcatac
//
