LOCUS       sequence-90041-e         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for Phic31 integrase-mediated transgene integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: pBT316_pattB-pCA-GFP-pA
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    19..140
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(30..49)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     84..103
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(307..328)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     656..675
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gggcgcgccc aatttaaata agatacattg atgagtttgg acaaaccaca actagaatgc
       61 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
      121 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
      181 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
      241 atgatcagtt atctagaatt cttacttgta aagctcgtcc atgccgagag tgatcccggc
      301 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
      361 ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
      421 ggtgttctgc tggtagtggt cggcgagctg cacgccgccg tcctcgatgt tgtggcggat
      481 cttgaagttc gccttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct
      541 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
      601 cttcagctcg atgcggttca ncagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
      661 gtagttgccg tcgtccttga agaagatggt gcgctcc
//
