LOCUS       sequence-90059-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  transgenic construct for expression of GFP and Flpo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    50..274
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     263..280
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgcc catagagccc
       61 accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc
      121 accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt
      181 tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg
      241 caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg agctctagat
      301 catctcatca gatccgcctg ttgatgtagc tgctcaggta gtccagcacc tcctggctga
      361 tgatgccgtt ccaggcgggg tatctgatgc tgccctcggc gctgcccttc agctgctcga
      421 tgtgctgcca ctcctcgatg gggttggtct cgtccttcag ggcgatcatc tccttgctga
      481 tggggtcgta ggcgtagtac ctggacacca gggcgaagta gtggtcgggg atggcggtga
      541 tctggtgggt gtaggtggtc ctggccacgg cggaggccct cttgtcgctc cagttgccca
      601 ccacgtttgt cagctcggtc aggcccttca tgctcagaaa gctggtcatc aggtgcctgc
      661 cgatgtggct cttagggccg ttcttgatag cgaagatggg gtagggggcg ttcttcttca
      721 gggccttgtt gtagctgcgc accaggttgt ccttcagcag ctggtactcc tgcttgttgc
      781 tgctgctgtt gccggtcctg ttcactctc
//
