LOCUS       sequence-90191-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIalpha R 1-2
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    6..30
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(116..421)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(218..237)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cttaggttta cccgccaata tatcctgtca aacactgata gtttaaacca gagaagacaa
       61 gcccgcggtg agaccgtcga cctgcagact ggctgtgtat aagggagcct gacatttata
      121 ttccccagaa catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag
      181 ccacttcttc cccgataacg gaaaccggca cactggccat atcggtggtc atcatgcgcc
      241 agctttcatc cccgatatgc accaccgggt aaagttcacg ggggacttta tctgacagca
      301 gacgtgcact ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata atatcactct
      361 gtacatccac aaacagacga taacggctct ctcttttata ggtgtaaacc ttaaactgca
      421 tttcaccagc ccctgttctc gtcagcaaaa gagccgttca tttcaataaa ccgggcgacc
      481 tcagccatcc cttcctgatt ttccgctttc cagcgttcgg cacgcagacg acgggcttca
      541 ttctgcatgg ttgtgcttac cagaccggag atattgacat catatatgcc ttgagcaact
      601 gatagctgtc gctgtcaact gtcactgtaa tacgctgctt catagcatac ctctttttga
      661 catacttcgg gtatacatat cagtatatat tcttataccg caaaaatcag cgcgcaaata
      721 cgcatactgt tatctggctt ttagtaagcc ggatcctcta gattacgccc cgccctgcca
      781 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaa
//
