LOCUS       sequence-9023-ex        5068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pDR3.06
FEATURES             Location/Qualifiers
     source          1..5068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3244..3343)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3455..3474)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3466..4275
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3533..3552)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4418..5006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4907..4926
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagcaggct ccatgtcgcc gcctctacgg tatcaaaaag accaacaaaa tcagcaacat
      481 cagcaaaatc aatcacagca agctgctcat cagatggtct ctttcatgcc acaaacaccg
      541 acttctcttc catcaactcc gatacaactt tatccaaccg gagcagccgc actaatacca
      601 gcaccaccta catacgagct gattccaaac cgtattttcg ttggtggctt cccaacttcg
      661 gttcgtgaat ttcttgatat gttctctgtt cttcctaact ctcgggttgt ttcagaccac
      721 agaaactgag ttgcgtgaac atttcgagaa attcttcgct gtgaaggacg tcaaaatggt
      781 taaatcactt gatggtcagt caaagggata cggatttatc accttcgaaa ccgaagatca
      841 agcagaggaa attcgtaaac tgactccaaa gcagttggaa ttccgtagca ggaagctcaa
      901 cctaggtcca gcgatccgaa aaataaactc caatagtttt caaccaagta agattttttt
      961 ctttaattct atagtgacta aaataaaaaa tttacaggct acgcaatcgc cactccaagt
     1021 cagctcgttg cggcatctcc aggaccattc agctacgcca ttccggcatc tccaagtcca
     1081 tatagcggat actcctatcc tgcttcgcct cagatgttcg tctatcctcc gttacgcagt
     1141 caggatcaaa gcaggcagca atctgagcaa caaactactc cgcagaatag tcctactaat
     1201 ctgcaacatc aacaatcgcc acaagtcttc ttcggtggag atcaagatcc aataagatct
     1261 tatgcgagtg cagttgcagg ttttttgtaa acaatttttt aaatctttta aatataattt
     1321 ttataggagt tgaaaagtcc gaagtttccc ctgaaaagca tgaatcagtt tctccacaac
     1381 cacttcttcc aaatcaaaat gtgttgaaca ctcagtattc ccaaggtcag cagcagtgga
     1441 attccaatgt gcagcagcag cagcaacaac aaatggactc gaacaacgga ggtccttact
     1501 ataatgaaaa ttattctcaa ggctacaccc gtcctcatcc atatcaacag tttgctcaat
     1561 ctggggtacg taatttccag attttacaaa gtagtagagt tcaataattt atcttttttt
     1621 taaccagttc gaatatataa tttcaggtgt acatgaacag ccaaggaatg taccattcga
     1681 gttattccta catgactcct ccactggcac caggtcaata tccacaaatg atgtccgctc
     1741 cctattggca acagcaacaa cagcaccatc aaagtcatgc atacgctggc tataatcccg
     1801 cctacaacaa ctgggttgga ccatctggag atatgaatca gttcaaacaa aatccgtcaa
     1861 actacttcta tcaaaattat ccgggaaatt tttcacaaca acacacgatg ggaaacaatg
     1921 aaaatacatt ctcccttccc ctccaagctc cacgccaagg caagaagagc cggaaaccat
     1981 cggaatgtca ggataagaag acaaaaagcc caatcaaagg ttagattttc gaaacagtgt
     2041 ttttattcaa gttttaaaag aaagtaatca tttttttaaa tattattctt attcttacag
     2101 gagctcgcac tgagagacca tcgtcatcag cttcgactcc agacgcgaaa tatcagaaga
     2161 accacagata tccagttcat ctttctccac tttcagcatc cctccaatca ctagccattt
     2221 cctcgcccac taagaataat taaatacgaa ttctacattt ttgtcattag tattttctgt
     2281 cctttaggta gttttctatt ccattgctct ggtgcctacc aaataagaat taaaacacgt
     2341 attttgtatc ctatacgcaa ctgtatattt gattcaaata ctagaattag ctagtttctt
     2401 tctctttttc ctatccaaat tatcttcctt acgaatattg ttccattcta taatgtgctt
     2461 ctgaagccat cgatcttttc atatcttcta gtaagattga atttagatcc cacgttcccc
     2521 attcccatag tattcaaatg tttttgtatg cccccaattt agaataaaat gaaatcacaa
     2581 taatgacacc agtagcccct ctctcgcatt acatggtaat tttgaatcac ccaaattgtc
     2641 cactttaaca cctaaaaaat ccgaagtaga attttgtgtg tgattttaat aaatcttttc
     2701 gcagagaaga ttgtgactta agtgtttttc ttattttttt ctgccgtgga ttatgttcat
     2761 aatccacaaa ttgttcaggc aaggtcgagg acatcgagtg aagcacaaaa attggtggat
     2821 ggttcacgga acactcacat cggaactcat tgatttcatg gaattctcaa ttccttcgtc
     2881 tttttaccgg aagcaatccc cttttcttat ccatccgata tcatcaattt cccttttcac
     2941 acgctaacaa ccttttcttt tctcggtgtc tcattgttcc atgatgtctc tcaaacgatc
     3001 ttcttttcat aacactttgc ctcatgttga ttgtgatttc aaagagagaa tcaaggcgat
     3061 ccgaccggcg atcggttctc gttttgagac atttttcgac ttttttcatt gtttgttcta
     3121 aaattataag agtttaaggg gtgaatatta aaaatcgcaa aaacaatttt aacttctgta
     3181 gctttagaaa tgcaaatcat ccggacacga aatttggtga ttattccgac aaaaatacgg
     3241 tatacccagc tttcttgtac aaagtgggca ttataagaaa gcattgctta tcaatttgtt
     3301 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgcagctc
     3361 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     3421 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     3481 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     3541 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     3601 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     3661 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     3721 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     3781 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     3841 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     3901 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     3961 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     4021 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     4081 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     4141 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     4201 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     4261 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     4321 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagttt tcgttccact
     4381 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     4441 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     4501 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     4561 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     4621 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     4681 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4741 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4801 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     4861 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4921 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4981 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5041 ccttttgctg gccttttgct cacatgtt
//
