LOCUS       sequence-90303-e        1175 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Miranda KC, Khromykh T, Christy P, Le TL, Gottardi CJ, Yap AS, Stow 
            JL, Teasdale RD
  TITLE     A dileucine motif targets E-cadherin to the basolateral cell surface
            in Madin-Darby canine kidney and LLC-PK1 epithelial cells.
  JOURNAL   J Biol Chem. 2001 Jun 22. 276(25):22565-72.
  PUBMED    11312273
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2001 Jun 22. 276(25):22565-72."
COMMENT     Sequence Label: E-cadherin-GFP
FEATURES             Location/Qualifiers
     source          1..1175
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        3..21
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3..20
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(82..103)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(238..750)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIE"
     primer_bind     431..450
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     690..711
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcatttaggt gacactatag aatagggccc tctagattac ttgatcagct cgtccatgcc
       61 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
      121 ggggtctttg ctcagggcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg
      181 gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc
      241 gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat
      301 atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc
      361 cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac
      421 ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta
      481 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct
      541 gaagcactgc acgccgtagg tcagggtggt cacgagggtg ggccagggca cgggcagctt
      601 gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc
      661 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
      721 caccccggtg aacagctcct cccccttgct ccgcgggtcg tcctcgccgc ctccgtacat
      781 gtcagccagc ttcttgaagc gattgcccca ttcgttcaag tagtcatagt cctggtcttt
      841 gtctgactct gaggagttca gggagctcag actagcagct tcggaaccgc ttccttcata
      901 gtcaaacacg agcagagaat cataaggcgg ggctgtgggg tcagtatcag ccgctttcag
      961 attttcatca ataaaatttc caatttcatc gggattggca gggcggggaa gataccgggg
     1021 gacactcatg agggttggtg caacgtcgtt acgagtcact tcaggccgag cgtccaggcc
     1081 cctgtgcagc tggctcaagt caaagtcctg gtcctcttct ccgcctcctt cttcatcata
     1141 gtaataaacg ttgtcccggg tgtcatcctc tgggg
//
