LOCUS       sequence-90314-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid vector carrying C-Bio-1xproteinA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Alekseyenko AA, Gorchakov AA, Kharchenko PV, Kuroda MI
  TITLE     Reciprocal interactions of human C10orf12 and C17orf96 with PRC2 
            revealed by BioTAP-XL cross-linking and affinity purification.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2488-93. doi: 
            10.1073/pnas.1400648111. Epub 2014 Feb 3.
  PUBMED    24550272
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1400648111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-02-18- 18"; volume: "111"; issue: "7"; pages: "2488-93"
COMMENT     Sequence Label: pCAndy-CBioTAP
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     193..215
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     207..224
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     208..224
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     234..253
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        234..252
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(282..455)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     primer_bind     complement(738..754)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(738..754)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(791..811)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(791..809)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
       61 gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
      121 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
      181 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg
      241 actcactata gggcgaattg ggtaccgggc ccctattata ctttcggcgc ctgagcacca
      301 tttagctttt tagcttctgc taaaaggtta gcgctttggc ttgggtcatc ttttaaactt
      361 tggatgaagg cgtttcgttg ttcttcgttt aagttaggta aatgtaagat ctcatagaac
      421 gcgttttgtt gttctttgtt gaatttgttg tccacctcga gtgacgatcc aacgccgatc
      481 ttgattagac cttgaccacc ttgaacagca tctctttcct taactaacac cttttcaact
      541 tttccgtcag taggagcgtt aatttctgtc tccattttca tagcctccag caccagaact
      601 gtttgaccag ctttaacagt gtcaccttct tttaccagta tcttagaaac ggtaccagca
      661 agaggggcag ggatttcacc ttcaccggcc tttccagccg aggagccgct ggcgcgccga
      721 attcctgcag cccgggggat ccactagttc tagagcggcc gccaccgcgg tggagctcca
      781 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
      841 t
//
