LOCUS       sequence-9036-ex        3141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.50
FEATURES             Location/Qualifiers
     source          1..3141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(624..747)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1250..1345)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1382..1401)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1383..1401)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1406..1422)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1406..1422)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1524..1543)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1535..2344
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1602..1621)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2491..3079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2980..2999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac
      601 taataccatc taagtagttg attcatagtg actgcatatg ttgtgtttta cagtattatg
      661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      721 ctcgttcaac tttcttgtac aaagtgggat aatcagttca actgatacgc ccattttatt
      781 tctactaact gcgtgttttg cattttatcg aacaccccta aactttcact taaccaaaaa
      841 gctttaacaa attttttacg gaatcttctt gtacagcgac actcgttgtc cttgtcaaat
      901 gattcgttgc tagtttttct tccctgtagc ttctatatcg acccccccgg tgttattttg
      961 taggaatgcg tttcagcatc ttccctattt tgccatctct cacatacact caacattagc
     1021 aatcggaaaa cagatcttct atattgaaaa tatactacca gtccgtttaa cctacctcgc
     1081 ttgctaacat tattgaatgt gcccacgtat tgtattttct gagcatcttt ctgttttttt
     1141 tgtattttat agacgctgtg ttcgccagtt atttttgcat tcctgtattg ctcgaagtca
     1201 aatgaagtgc ttcgccatat tcaattgttt tattttcaaa atcacccgtc aactttatta
     1261 tacaaagttg gcattataaa aaagcattgc ttatcaattt gttgcaacga acaggtcact
     1321 atcagtcaaa ataaaatcat tatttggagc tccatggtag cgttaacgcg gccgcgatat
     1381 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     1441 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
     1501 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
     1561 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     1621 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
     1681 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     1741 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     1801 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
     1861 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     1921 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     1981 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     2041 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
     2101 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     2161 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     2221 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     2281 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     2341 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga
     2401 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     2461 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2521 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2581 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     2641 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2701 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2761 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2821 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2881 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2941 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3001 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3061 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3121 ctggcctttt gctcacatgt t
//
