LOCUS       sequence-90417-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 6, small linker insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_6_Linker
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          71..643
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     promoter        complement(740..758)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(741..758)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cgatccgtcg aggaattaat tctttgccaa aatgatgaga cagcacaaca accagcacgt
       61 tgcccaggag ctgtaggaaa gagaagaagg catgaacatg gttagcagag gggcccggtt
      121 tggactcaga gtattttatc ctcatctcaa acagtgtata tcattgtaac cataaagaga
      181 aaggcaggat gatgaccagg gtgtagttgt ttctaccaat aagaatattt ccacgccagc
      241 cagaatttat atgcagaaat attctacctt atcatttaat tataacaatt gttctctaaa
      301 actgtgctga agtacaatat aatataccct gattgccttg aaaaaaaagt gattagagaa
      361 agtacttaca atctgacaaa taaacaaaag tgaatttaaa aattcgttac aaatgcaagc
      421 taaagtttaa cgaaaaagtt acagaaaatg aaaagaaaat aagaggagac aatggttgtc
      481 aacagagtag aaagtgaaag aaacaaaatt atcatgaggg tccatggtga tacaagggac
      541 atcttcccat tctaaacaac accctgaaaa ctttgccccc tccatataac atgaatttta
      601 caatagcgaa aaagaaagaa caatcaaggg tccccaaact caccctgaag ttctcaggat
      661 cggtcgagat ccttacttag ttacccggga ggatgagggt ctcaaactgg taccgccgcc
      721 ggatccgcct gatgagacct ctatagtgtc acctaaatca agcttgctcc gacgtcccca
      781 ggcagaatgg cggttcccta aacgagcatt gcttatatag acctcccatt aggcacgcct
      841 accgcccatt tacgtcaatg gaacgcccat ttgcgtcatt gccc
//
