LOCUS       sequence-90470-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya-C1
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..749
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSFLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMYLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
     misc_feature    750..815
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggggc
       61 aatcgaagca tgggctaaag gaggagatga cggtgaagta ccacatggaa ggttgcgtta
      121 atggtcacaa atttgtcatt actggagagg gcattggaaa cccttttaag ggtaagcaaa
      181 ctgcaaattt gtgtgtgata gaaggaggcc cgctgccgtt ctcggaggac attttaagtc
      241 cgggttttaa atatggtgac cggattttca cagagtaccc acaggatatt gtagattact
      301 tcaagaactc atgtccagcg ggctatacgt gggaaaggag cttcctcttt gaggacggag
      361 cggtctgtcg atgcaacgtg gacataacag tctctgaaaa ggagaactgc atctatcaca
      421 aaagtatctt cagaggggtg aattttcccg ccgacggccc cgtaatgaag aaaatgacca
      481 ctaattggga agctagtacc gagaaaattg tgcctgttcc aaagcaaggg atattaaaag
      541 gaaaggtcaa aatgtaccta ttgctgaagg atggcggtcg ttatcattgc cagtttgata
      601 cggtatataa agctaaatca gtgccctcca agatgccaga atggcatttt atacagcata
      661 agctccttag ggaggaccgc tctgatgcta aaaaccagaa atggcaatta acagaacatg
      721 caatcgcagg catggacgag ctgtacaagt ccggactcag atctcgagct caagcttcga
      781 attctgcagt cgacggtacc gcgggcccgg gatccaccgg atctagataa ctgatcataa
      841 tcagccatac cacatttgta gaggtt
//
