LOCUS       sequence-9053-ex        4138 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM3.19
FEATURES             Location/Qualifiers
     source          1..4138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      1990..2313
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(2314..2413)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2525..2544)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2536..3345
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2603..2622)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3488..4076
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3977..3996
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagcaggct ccatgagaga gatcgtccac gtgcaagccg gacaatgcgg aaaccaaatc
      481 ggatccaaat tctgggaggt catctccgac gagcacggaa tccagccaga cggaaccttc
      541 aagggagaga cggatctcca gcttgagcgc attgacgtct actacaacga agccaacagt
      601 gagtttttaa aattttctgg agatttcgaa atcgtttagt tggaatgatt aattaattaa
      661 attttttcag acggaaagta tgtgccacgc gccgtgttgg tcgatctcga accaggaacc
      721 atggattctg tccgctctgg accattcggc cagctgttcc gtcctgacaa ctttgtgttc
      781 ggacaaagcg gagccggaaa caactgggcc aagggtcact acacagaagg agctgagctt
      841 gtcgacaatg tgcttgacgt gatccgcaag gaagccgaag gatgcgattg tcttcaagga
      901 ttccaactca ctcactctct cggaggagga accggatctg gaatgggaac acttctcatc
      961 tccaaaatcc gcgaagagta cccagacaga atcatgagct cattctcggt tgtaccatcg
     1021 ccaaaggtgt ccgacacagt tgtagaacca tacaatgcca ccctctccgt ccaccagctt
     1081 gttgagaaca ccgatgagac ctactgcatt gacaacgagg ccctctacga catctgctac
     1141 cgtaccctca aactcaccaa cccaacctac ggtgatttga accatcttgg taagttatta
     1201 tttcttgttc aagtttaagt tatgcacaag ttacccaaat tgaaacctaa taattacaat
     1261 tttcagtatc cctcaccatg tccggagtta ccacctgtct ccgattccca ggacagctca
     1321 atgccgatct ccgcaagctt gccgtcaaca tggttccatt cccacgtctt cacttcttca
     1381 tgccaggatt cgcaccactt tccgccaagg gaacccaggc ttaccgtgcc cttaccgtcg
     1441 ccgagctcac ccaacaaatg ttcgatgcca agaacatgat ggctgcctgt gacccaagac
     1501 acggacgtta cctgaccgtg gccgctatgt tccgtggacg catgtcgatg agagaggttg
     1561 acgagcaaat gctcaacgtt cagaacaaga actcgtccta cttcgtcgaa tggatcccga
     1621 acaacgtcaa gaccgccgtt tgtgacatcc caccaagagg actcaagatg gctgccacct
     1681 tcgttggaaa ctcgaccgct atccaagagc ttttcaagcg tatctcagag cagttcactg
     1741 gtaagaaatt tgaaactatc atccagtatc agttttaaaa acgttatttt ttcagctatg
     1801 ttccgccgca aggctttcct ccattggtac actggagaag gaatggacga gatggagttc
     1861 accgaagccg agagcaacat gaacgatctc atctcagaat accaacaata ccaagaagca
     1921 actgccgaag acgacgtcga cggatacgct gagggagaag ctggagagac ttacgaatcg
     1981 gagcaataaa tgcaagatcc tttcaagcat tcccttcttc tctatcactc ttctttcttt
     2041 ttgtcaaaaa attctctcgc taatttattt gcttttttaa tgttattatt ttatgacttt
     2101 ttatagtcac tgaaaagttt gcatctgagt gaagtgaatg ctatcaaaat gtgattctgt
     2161 ctgatgtact ttcacaatct ctcttcaatt ccattttgaa gtgctttaaa cccgaaaggt
     2221 tgagaaaaat gcgagcgctc aaatatttgt attgtgttcg ttgagtgacc caacaaaaag
     2281 aggaaacttt attgtgccgc caagaaaaaa gtcacccagc tttcttgtac aaagtgggca
     2341 ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     2401 aaatcattat ttgccatcca gctgcagctc tggcccgtgt ctcaaaatct ctgatgttac
     2461 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     2521 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     2581 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     2641 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     2701 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     2761 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     2821 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     2881 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     2941 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     3001 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     3061 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     3121 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     3181 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     3241 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     3301 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     3361 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     3421 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3481 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3541 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3601 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     3661 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3721 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3781 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3841 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3901 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3961 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4021 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4081 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
