LOCUS       sequence-9054-ex        5090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM3.29
FEATURES             Location/Qualifiers
     source          1..5090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3266..3365)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3477..3496)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3488..4297
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3555..3574)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4440..5028
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4929..4948
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagcaggct ccatgacaac tcacctaccg agcacatctc aaaatggcga agagatatct
      481 gctgagcaat tcaacaggtg atttatttaa tttaataaca atttaatcaa ttttttattt
      541 tcaggatatt tcacgaacgc aacgtcatcg ttctggattg tcgatcaaat ggtgattcag
      601 tgaaacgagc caatcggtta gtattttaaa tttatttttc tcgtaatcaa aaaatctaaa
      661 aaccttctaa ataataaaca atctcggtgt agattctttt gcagtcttcg attaccggct
      721 ctccttcaac gccgtttaat gggcggttca atgcgacttt caacagtacc tgatctgaag
      781 gatctgaatg tgagtttatt attattattt ttacacattt atttcaataa aaacggacag
      841 aaaacattaa aattgttcga aaaatactga aaatcatttt cagaattctc ctgatcaatg
      901 tcccgaagtt cttctcatcc ccggcgacag tgaacaagac gagcagctct cggcagcttt
      961 agctcgaaat ctgaaatcca accactatcg tcattttgtg ctcggcgagc ctgtcaagac
     1021 acttctctcc cagtttccga cccttcgtga tgcagccgac gaaaattgga acaccacgtt
     1081 tcaaatgaac tcgatgccgg gtcaagcaag tgggcaacaa gcatcaagtg gaccgcttct
     1141 taacttaaag ttagtttttt taattaaaaa cttattaagt tatttctaat ttttttaaat
     1201 tataaaaatt aaagattcga tccgtgaatt ttgattgtga agaaatttca tgttttcaca
     1261 gccaaattag catgaaatat cttttcaatc ttatacgaat taattttcat tcgaaaaagg
     1321 aaataaatca agcaatatcg aaactttatt gaaaaaataa acgaaaaagc agtggaaatc
     1381 ggcccgactc tatttctgta cgacgctgct ttttttcaaa atttcccaga tcgatccttt
     1441 aattttaaca gtataaccag gtttgaagct ccaaaaatgc atgtattgtt aattgaaaaa
     1501 aattccagaa aacagtagat aatttttatt ttccagccaa ctccgattgg aaggtgaaga
     1561 tcaaggcgga aaacaacgtg cagagtttcc tgtaaagtta accaatttct tgtatcttgg
     1621 aaacgcggag accgctaaaa atcgagatgt tcttaaaaag tgacactatt attcgatatt
     1681 atatatattc ttaacaaaaa acccaatttt cagatattct atctcacatg taataaatgt
     1741 cacatcaaat ttgccaaaca cgttcgaaga agacccaaat atgcgatatt taagaatatc
     1801 ggccgatgat aacgcttcac ataatctaac aaaattcttc cctggtttgt aattgatttt
     1861 tctgagtttt cttaaaaata ttccttttca gaagccatat ccttcattga tgatgcgcga
     1921 cgcaacgatt cagcatgtct tgtgcattgt ctggctggaa tttccagaag cgttacaata
     1981 tgtctggcgt atctcatgaa gactgaaatg taatttgatt tagattttat agattaaaaa
     2041 aaattgatat ttttcaggtg tactcttgat tctgcctacg aatgggttca aaagagaaat
     2101 gcaagcattg ctccaaattt tcattttatg ggacaactca cagactacga gaagatgttg
     2161 ggattgaata gcaatcgagt cggggttagt aggcaagctg taaattttga tgttcacttt
     2221 ttctgcattt ttaaattaaa gaaaaaagag gagaactccc cagaatcaag aattttagcc
     2281 gaaactgcag tttaaactgt aaaatcgtgc agaaaaatac atactgtgtt gttcacgtgt
     2341 agtacaccgt aaatcgacac aggttgctgc ctttgtggtt caaatcctac ccgttgattg
     2401 cttatccgtt agaatatttt aataaatcgt ccttcttcta caaatgttct tttagtgatt
     2461 ttttcgtatt tcagacatat ccttcctcgg ctcctcgttc tccatcttgt gccatcgaag
     2521 cagcagcagt atcacaagtt ggaggccttc taactccacc accaacaagc tgttcggcgt
     2581 cacctcaatc gagtaatcac tcggcaaagt catttcacta gaagaattca ccccttctta
     2641 taatttttca tcatattttt tcatccattt ttatcgtcct catgcagcta gatgtacaaa
     2701 taatacaact ctactacctg ttatgtttcc tagttgtcat accattgtga tattctttct
     2761 ttttggtaga tccacttcct gtcccatgac tgtccttttt tatgtctcga cgttcttttc
     2821 tcttgtgaaa tgaccctacc atgatgcctg ttttaccaaa tcaataattt taaaccacca
     2881 ccaccacaac gacacgtgtc tctttccctt ttcattaccg tatagttcat ttcccccact
     2941 acgacacttt ccatacaatc tctgtacctt atttttctct catttgtcag actttcaaaa
     3001 ttgagttttc tctttttccc atcatctccc ctaaaaccac tttcccgaga aaatccgcaa
     3061 cattgttatt gccatgacct tacttttccc tcccaattct caatcagcac caccatttcc
     3121 tgctatcaca ctttctcccc attcactgat ttcagcctcg tctaattcac ctaaattttc
     3181 ataattgttc tcgattttat ctttttcttc tctcattcaa atcaccgact ctgaactttt
     3241 tagtgaacgc cgagtctttg attccaccca gctttcttgt acaaagtggg cattataaga
     3301 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     3361 atttgccatc cagctgcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     3421 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     3481 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga
     3541 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     3601 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     3661 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     3721 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     3781 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     3841 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     3901 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     3961 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     4021 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     4081 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     4141 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     4201 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     4261 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
     4321 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct
     4381 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4441 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4501 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4561 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     4621 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4681 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4741 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4801 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4861 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4921 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4981 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5041 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
