LOCUS       sequence-9058-ex        5427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM3.09
FEATURES             Location/Qualifiers
     source          1..5427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3603..3702)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3814..3833)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3825..4634
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3892..3911)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4777..5365
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5266..5285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagcaggct ccatggacca atcaaagatg cgcagaacga atcagttcag aaaagtaagt
      481 tttcagtaac ttctaaagaa acaaatggcc taagttttac agacttccca gaagcctcct
      541 tctacaggga ttgacagtta tccgactccc gcacaatcac caatggcgca gcatgagaca
      601 cctatgtggg acttcaactc tctcaacccg tatttctcaa tgctcaatat gaacgatggg
      661 attaattatg ctcgccatca acagaatcac attgttacat cgcggccacc aaccccgcta
      721 actgatttga tgagccttcg gagttttcag agctttgtaa gttagagtag gcaatttttg
      781 gttgttttca ttactgattt gcagcccaat gtgttcatgc cggtctcgcg tagccgaact
      841 tcatcattca ttcagtctga tacggattcc tcgcgtctcg agtcggatga tttttcacaa
      901 aatgtccgat gtttctctgc ggatattgat cgtagcaaga gctatggttc ttcaaaacac
      961 taccatttga aatattcgag gttcgaatta atttatcact atcctctagt atttattctt
     1021 ttttatttat agacccgctc tgtcgaggaa ctctagatca ttcacaagat caaacaatgt
     1081 gctgccaacc tggtccttgg attccaatgg tgaaatgcgt tcaagattgt cgctctctga
     1141 agttttggat agtggcgatt taatgaagtt tgccgttgac aaaactgggt gccagttcct
     1201 agaaaaggca gtgaagggca gcctgacatc ttatcagaag ttccaattgt ttgaacaagt
     1261 tattgggaga aaagatgatt tccttgtaag ttttagtttc tgcgagcaca catgactttt
     1321 cttttcagaa attgagcacg aacatctttg gaaattattt ggtacaaagc gttattggaa
     1381 tttcattggc aactaatgat gacggataca caaagcgaca agaaaagttg aagaacttca
     1441 tcagttccca aatgacagat atgtgcttgg acaaatttgc gtgtcgtgtt atccaatcca
     1501 gtctccagaa catggattta tcccttgcgt gtaaacttgt tcaagctcta cctcgtgatg
     1561 ctcgtcttat cgcaatctgt gttgatcaaa atgccaatca cgtcattcag aaagtcgtag
     1621 cagtcatccc actgaagaat tgggagttta ttgtggattt cgttgccacc ccagagcacc
     1681 tccgtcagat ttgttctgat aagtatgggt gccgtgttgt gcaaactatt atcgaaaagc
     1741 tcactgctga ttcaatgaac gttgatttaa cttccgctgc tcaaaatctt cgtgaacgag
     1801 cgctccaacg actcatgact tctgtgacta atcggtgcca agaattagct acgaacgagt
     1861 atgccaatta cataatacag catattgtat ccaatgatga tctggccgtt tatcgggaat
     1921 gtatcataga gaaatgcctc atgtgagtgt tattgtttaa tatctacttg atttttaatc
     1981 atcaatattt caggcgcaac cttctttcgt tgtcccagga aaagtttgct tctcatgttg
     2041 ttgagaaagc attcctacat gcaccattgg aacttcttgc cgaaatgatg gacgagattt
     2101 tcgacggata cattccacat ccgtaaaaac attagtttca gtcacataaa aatgaaatgt
     2161 tttttcaggg acacgggaaa agatgccctc gatatcatga tgttccatca attcggaaac
     2221 tacgtcgttc aatgcatgct gactatttgc tgtgatgctg tgtctggaag acggcaaacg
     2281 aaagaaggag gctatgacca tgcaatctca ttccaggatt ggctgaaaaa gttgcactcg
     2341 agagttacga aagaaaggca tcgcttgtca aggttatcac tattgtcaca ataatatttt
     2401 gaaattcgaa attcaggttc tcatccggca aaaagatgat cgagacgctt gccaatctcc
     2461 gttcaactca tccgatttat gaactccaat cttcgggaca cgattcattc aagacggatt
     2521 acttcagtac tgcttccgag cacgatggtc cggagttgga gaagaatggg atcgaggaag
     2581 gaagcctgat gctagagcca cggagcaata agtcttcagt ctctgtcaaa ttttcttctt
     2641 ccggtagtca cggcgatgat taaggtggaa ctttctcacc ataaatctca tcccacccac
     2701 ttatgttctg ttgttttgta atattttaaa ctggtgcaat gatttcgttt cgattttaag
     2761 atcgatgttg tgccttcccc actcttatca ttgcccagtt ttgttttttc ttttcttttt
     2821 tgttatttta ttgcttttta tgcagttgtg ttattgtttc tttctgttta ctgttcattg
     2881 tttttgttaa ttttttattt tcgattttaa tgaatccaga taaaattaga caacttggca
     2941 aaatatcgtt cgagtctttg tcatgcttcc accgagagct tcctgcttcc atacaatgcc
     3001 tgctcagaaa acaattttga aagcgaagag aaaagttgtt ctttgctcgg agaacaaaaa
     3061 actgataacc gacaaatgtt gactttgtaa atttaatcaa attagaaacc atgaagaata
     3121 aatatagttc aattatcata ttgaaattta tcttacaatt gcatcgattt caaaataatg
     3181 ggactctcag aaaatagaaa aacaaaaatt ggatactggg gatgaggaag aactccgcga
     3241 acattggcgg tccttctctc atgtggtcct cgtataggtg gtggcaatcc tctcattgtt
     3301 atccaacatc gagaaaatgt tcttcggcta gaactctgaa acaaaaatac tttcaaatta
     3361 aaattgtcaa gataaattcg tacttcctga cacaatttgt gttgttgcag cgttgaattc
     3421 gattccaaac caagtggcaa gaccaagacc cagcaatgat gatggaatag ttgaattgac
     3481 agaattcctc gagcatatca gcaatggatg ttgatgcaag tggatgcaat gttcttcttt
     3541 tgtttctcat caacgtttgc cttatgattg tgaagtgact tgatgaactc tctctgccat
     3601 gcacccagct ttcttgtaca aagtgggcat tataagaaag cattgcttat caatttgttg
     3661 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgcagctct
     3721 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
     3781 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
     3841 gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     3901 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg
     3961 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     4021 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
     4081 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc
     4141 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     4201 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     4261 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     4321 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat
     4381 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     4441 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     4501 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     4561 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     4621 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga
     4681 cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4741 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4801 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4861 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4921 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4981 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5041 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5101 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5161 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5221 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5281 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5341 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5401 cttttgctgg ccttttgctc acatgtt
//
