LOCUS       sequence-9065-ex         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Zhou Q, Brown J, Kanarek A, Rajagopal J, Melton DA
  TITLE     In vivo reprogramming of adult pancreatic exocrine cells to 
            beta-cells.
  JOURNAL   Nature. 2008 Oct 2;455(7213):627-32. doi: 10.1038/nature07314. Epub 
            2008 Aug 27.
  PUBMED    18754011
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07314"; journalName: "Nature"; date: "2008-10-2- 2"; 
            volume: "455"; issue: "7213"; pages: "627-32"
COMMENT     Sequence Label: pAd nGFP
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             69..89
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             93..113
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(123..125,126..128,129..641)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(168..189)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(429..448)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcanagtttg tncnnnngca ggctccncca tggtgcacgt ggatccnaan nnnannannn
       61 nggnagatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtacacgtgt
      121 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      181 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      241 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      301 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
      361 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      421 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      481 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      541 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
      601 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
      661 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
      721 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacntgg
      781 ncctgctgga gttcgtgacc gccnc
//
