LOCUS       sequence-90666-e        1603 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pDONR P2r-P3 carrying the STE3 promoter mCherry fusion (reverse 
            orientation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1603)
  TITLE     Fluorescent X-QTL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: P2r-P3 Ste3pmCherry
FEATURES             Location/Qualifiers
     source          1..1603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    6..26
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1012..1030)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1280..1299
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    1577..1601
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggggacaact ttgtataata aagttgccaa gttacctaag aggtgtacga tatttttttc
       61 ttttatatat atgattttct ctattcattt tttagttttt tttgatacat aagcgaatcg
      121 cacattgcgc aacttcaatt tgttgattcg ccaaagtatt cttaccataa aacaaccatt
      181 cgttgcttta ccctttcgta atcatttacc gtgataacca taatcagaaa cttattattt
      241 cagcctagta gaccggccaa gcaggccttg taatgtttct cttgattgct tgaatctttt
      301 aagcagccaa atctttccaa aaaaatgcaa ttatcagaac aaaactattt aaggtgactt
      361 ctccgtattt acaccaccag aagcgttctg gctccccttt tctctaaacg ttaaacattt
      421 tacaattgaa atgttaccaa tcctatatta ttgtaccaca ttgccagatt tatgaactct
      481 gggtatgggt gctaattttc gttagaagcg ctggtacaat tttctctgtc attgtgacac
      541 taattaggaa acttctcgac tatcaatgtg taaatgaagg aataatggcg gaaactttga
      601 aactttgtca ataattgcat cattggatgc gtttcatttg gccgttatca cggagaggca
      661 gagttctctc cacaatttgg gcagaagtct tttgaaaaga catatatata tatatatatg
      721 tatatgagtg gatgcttaag gtaagaataa tttctgaatt cccaagtatt cattttgtgc
      781 agtattcaca tattctattt tattgctttt taactttaga ggcaattaaa tttgtgtagg
      841 aaaggcaaaa tactatcaaa attttcatgg tgagcaaggg cgaggaggat aacatggcca
      901 tcatcaagga gttcatgaga ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
      961 tcgagatcga gggcgagggc gagggcagac cctacgaggg cacccagacc gccaagctga
     1021 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     1081 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     1141 tccccgaggg cttcaagtgg gagagagtga tgaacttcga ggacggcggc gtggtgaccg
     1201 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ttgagaggca
     1261 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     1321 ccgagagaat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     1381 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     1441 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     1501 actacaccat tgtggaacaa tatgaaagag ctgaaggtag acattctact ggtggtatgg
     1561 atgaattgta caaataacca ctttgtacaa gaaagctgtc ccc
//
