LOCUS       sequence-90667-e         298 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pDONR221 carrying the Cyc1 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 298)
  TITLE     Fluorescent X-QTL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pD221 Cyc1_term
FEATURES             Location/Qualifiers
     source          1..298
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    5..29
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      29..276
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(45..63)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     protein_bind    complement(270..294)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggggacaagt ttgtacaaaa aagcaggctc atgtaattag ttatgtcacg cttacattca
       61 cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag
      121 gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt
      181 tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt
      241 gagaaggttt tgggacgctc gaaggcttta cccagctttc ttgtacaaag tggtcccc
//
