LOCUS       sequence-90705-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 406, Emission: 526.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Ai HW, Hazelwood KL, Davidson MW, Campbell RE
  TITLE     Fluorescent protein FRET pairs for ratiometric imaging of dual 
            biosensors.
  JOURNAL   Nat Methods. 2008 May;5(5):401-3. doi: 10.1038/nmeth.1207.
  PUBMED    18425137
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2008-05"; volume: "5"; issue: 
            "5"; pages: "401-3"
COMMENT     Sequence Label: mAmetrine-C1
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      49..58
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(55..57,58..60,61..771)
                     /codon_start=1
                     /product="yellow fluorescent protein with a large Stokes 
                     shift, excitable with violet light (Ai et al., 2008)"
                     /label=mAmetrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTSGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GSYRTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMNVWDAYITADKQKNGIK
                     ANFKIEHNVEDGGVQLADAYQQNTPIGDGSVLLPDNHYLSFQSKLFKDPNEQRDHMVLL
                     EFVTAAGITPGMDELYK"
     primer_bind     complement(100..121)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    772..837
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 tagcagagct ggtttagtga accgtcagat ccgctagcgc taccggtcgc caccatggtg
       61 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      121 gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag
      181 ctgaccctga agttcatctg cacctccggc aagctgcccg tgccctggcc caccctcgtg
      241 accaccctgt cttacggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
      301 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat ctccttcaag
      361 gacgacggca gctacaggac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      421 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      481 gagtacaaca tgaacgtgtg ggacgcgtat atcacggccg acaagcagaa gaacggcatc
      541 aaagcgaact tcaagatcga gcacaacgtc gaggacggcg gcgtgcagct cgccgacgcg
      601 taccagcaga acacccccat cggcgacggc tccgtgctgc tgcctgacaa ccactacctg
      661 agcttccaga gcaagctgtt caaagacccc aacgagcagc gcgatcacat ggtcctgctg
      721 gagttcgtta ccgccgccgg gatcactccc ggcatggacg agctgtacaa gtccggactc
      781 agatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc
      841 ggatctagat a
//
