LOCUS       sequence-90716-e       10065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Derived from HSC1-GiP where 2 copies of the HS4 insulator core 
            inside the 3'LTR and the D4Z4-c insulator, are combined upstream of 
            the EF1 alpha promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10065)
  AUTHORS   Rival-Gervier S, Lo MY, Khattak S, Pasceri P, Lorincz MC, Ellis J
  TITLE     Kinetics and epigenetics of retroviral silencing in mouse embryonic 
            stem cells defined by deletion of the D4Z4 element.
  JOURNAL   Mol Ther. 2013 Aug;21(8):1536-50. doi: 10.1038/mt.2013.131. Epub 
            2013 Jun 11.
  PUBMED    23752310
REFERENCE   2  (bases 1 to 10065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/mt.2013.131"; journalName: "Mol Ther"; date: "2013-08"; 
            volume: "21"; issue: "8"; pages: "1536-50"
COMMENT     Sequence Label: HSC1-HS4-D4Z4c-GiP
FEATURES             Location/Qualifiers
     source          1..10065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        2815..3997
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3046..3988
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3945..3965
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(4007..4009,4010..4012,4013..4726)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4007..4009,4010..4012,4013..4723)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4052..4073)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4313..4332)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4660..4681
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(4767..5340,5341..5343,5344..5352)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4934..4951)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5161..5180
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5353..5952
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5353..5372)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5849..5869
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    6133..6166
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7110..7126)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7110..7126)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7123..7145)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7134..7150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7158..7164,7165..7182,7183..7188))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7203..7224
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7341..7358)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7512..8100)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7592..7611)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8271..9062,9063..9131))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8894..8913
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9132..9236)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9304..9322
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9360..9382)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9482..9501
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9695..9717
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9845..9864
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(10064..10065,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaattc gttcctcctg gctgcacctg ccgcagtgca
     1441 cagtccggct gaggtgcacg ggagcccgcc ggcctctctc tgcccgcgtc cgtccgtgaa
     1501 attccggccg gggctcaccg cgatggccct cccgacaccc tcggacagca ccctccccgc
     1561 ggaagcccgg ggacgaggac ggccacggag actcgtttgg accccgagcc aaagcgaggc
     1621 cctgcgagcc tgctttgagc ggaacccgta cccgggcatc gccaccagag aacggctggc
     1681 ccaggccatc ggcattccgg agcccagggt ccagatttgg tttcagaatg agaggtcacg
     1741 ccagctgagg cagcaccggc gggaatctcg gccctggccc gggagacgcg gcccgccaga
     1801 aggccggcga aagcggaccg ccgtcaccgg atcccagacc gccctgctcc tccgagcctt
     1861 tgagaaggat cgctttccag gcatcgccgc ccgggaggag ctggccagag agacgggcct
     1921 cccggagtcc aggattcaga tctggtttca gaatcgaagg gccaggcacc cgggacaggg
     1981 tggcagggcg cccgcgcagg caggcggcct gtgcagcgcg gcccccggcg ggggtcaccc
     2041 tgctccctcg tgggtcgcct tcgcccacac cggcgcgtgg ggaacggggc ttcccgcacc
     2101 ccacgtgccc tgcgcgcctg gggctctccc acagggggct ttcgtgagcc aggcagcgag
     2161 ggccgccccc gcgctgcagc ccagccaggc cgcgccggca gagggggtct cccaacctgc
     2221 cccggcgcgc ggggatttcg cctacgccgc cccggctcct ccggagccgg ggcgctctcc
     2281 caccctcagg ctcctcggtg gcctccgcac ccgggcaaaa gccgggagga ccgggacccg
     2341 cagcgcgacg gcctgccggg cccctgcgcg gtggcacagc ctgggcccgc tcaagcgggg
     2401 ccgcaggcca aggggtgctt gcgccaccca cgtcccaggg gagtccgtgg tggggctggg
     2461 gccggggtcc ccaggtcgcc ggggcggcgt gggaacccca agccggggca gctccacctc
     2521 cccagcccgc gcccccggga cgcctccgcc tccgcgcggc aggggcagat gcaaggcatc
     2581 ccggcgccct cccaggcgct ccaggagccg gcgccctggt ctgcactccc ctgcggcctg
     2641 ctgctggatg agctcctggc gagcccggag tttctgcagc aggcgcaacc tctcctagaa
     2701 acggaggccc cgggggagct ggaggaacaa gctttgcaaa gatggataaa gttttaaaca
     2761 gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc
     2821 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     2881 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     2941 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     3001 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
     3061 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
     3121 acgcccctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
     3181 gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc
     3241 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
     3301 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
     3361 agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc
     3421 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
     3481 gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg
     3541 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     3601 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac
     3661 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     3721 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     3781 gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     3841 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     3901 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     3961 ggttcaaagt ttttttcttc catttcaggt gtcgtgagaa ttagccatgg tgagcaaggg
     4021 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     4081 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     4141 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     4201 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     4261 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     4321 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     4381 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     4441 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     4501 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     4561 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     4621 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     4681 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccaacctcg
     4741 acgttattcc cttcgatcga attccgcccc tctccctccc ccccccctaa cgttactggc
     4801 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     4861 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     4921 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     4981 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     5041 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     5101 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     5161 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     5221 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     5281 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat
     5341 atggccacaa ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     5401 ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     5461 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     5521 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     5581 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     5641 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     5701 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     5761 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     5821 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     5881 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     5941 cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
     6001 catgcatcga tcatatggat aaaataaaag attttattta gtctccagaa aaagggggga
     6061 atgaaagacc ccacctgtag gtttggcaag ctaggagctc tcccatatgg tcgacggtat
     6121 cgccggggat ctataacttc gtataatgta tgctatacga agttatagat cctctagcga
     6181 taagcttgat atcgaattcg agttggcgcg cctgtcattc taaatctctc tttcagccta
     6241 aagctttttc cccgtatccc cccaggtgtc tgcaggctca aagagcagcg agaagcgttc
     6301 agaggaaagc gatcccgtgc caccttcccc gtgcccgggc tgtccccgca cgctgccggc
     6361 tcggggatgc ggggggagcg ccggaccgga gcggagcccc gggcggctcg ctgctgcccc
     6421 ctagcggggg agggacgtaa ttacatccct gggggctttg ggagggggct gtccccgtga
     6481 gctcccaggc gcgcctgtca ttctaaatct ctctttcagc ctaaagcttt ttccccgtat
     6541 ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg
     6601 tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga
     6661 gcgccggacc ggagcggagc cccgggcggc tcgctgctgc cccctagcgg gggagggacg
     6721 taattacatc cctgggggct ttgggggggg gctgtccccg tgagctcaat aaaagagccc
     6781 acaacccctc actcggcgcg ccagtcctcc gatagactgc gtcgcccggg tacccgtgta
     6841 tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc
     6901 tcctctgagt gattgactac ccgtcagcgg gggtctttca tgggtaacag tttcttgaag
     6961 ttggagaaca acattctgag ggtaggagtc gaatattaag taatcctgac tcaattagcc
     7021 actgttttga atccacatac tccaatactc ctgaaatagt tcattatgga cagcgcagaa
     7081 gagctgggga gaattaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7141 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7201 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7261 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7321 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7381 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7441 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7501 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7561 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7621 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7681 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7741 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7801 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7861 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7921 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     7981 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8041 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8101 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8161 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8221 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8281 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8341 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8401 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8461 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8521 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8581 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8641 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8701 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8761 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8821 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8881 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8941 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9001 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9061 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9121 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9181 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9241 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     9301 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     9361 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     9421 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     9481 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     9541 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
     9601 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     9661 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     9721 gcgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
     9781 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
     9841 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
     9901 ccacgatgcg tccggcgtag aggcgattag tccaatttgt taaagacagg atatcagtgg
     9961 tccaggctct agttttgact caacaatatc accagctgaa gcctatagag tacgagccat
    10021 agataaaata aaagatttta tttagtctcc agaaaaaggg gggaa
//
