LOCUS       sequence-90726-e         153 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA 
            intein, flanked by ITRs for AAV packaging. Combine with C-Terminal 
            Split Cas9 Gyra Intein for full length SpCas9 nickase production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 153)
  AUTHORS   Fine EJ, Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao G
  TITLE     Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human 
            cells using compact expression cassettes.
  JOURNAL   Sci Rep. 2015 Jul 1;5:10777. doi: 10.1038/srep10777.
  PUBMED    26126518
REFERENCE   2  (bases 1 to 153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-07-1"; volume: "5:10777. doi"; pages: " 
            10.1038/srep10777"
COMMENT     Sequence Label: N-Terminal Split Cas9 D10A Nickase with GyrA i...
FEATURES             Location/Qualifiers
     source          1..153
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   7..147
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   18..147
                     /label=AAV2 ITR
ORIGIN
        1 acatgtcctg caggcagctg cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg
       61 ggcgtcgggc gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag
      121 tggccaactc catcactagg ggttcctaca tgt
//
