LOCUS       sequence-90775-e        1473 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Characteristic fingerprint molecule to perform single molecule force
            spectroscopy experiments; covalently immobilized via ybbr tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash 
            MA, Gaub HE
  TITLE     From genes to protein mechanics on a chip.
  JOURNAL   Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099.
  PUBMED    25194847
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099."
COMMENT     Sequence Label: pET28a-ybbR-HIS-Xyl-DocI
FEATURES             Location/Qualifiers
     source          1..1473
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..66
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             73..90
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggtaccg actctctgga attcatcgct tctaaactgg ctctggaagt tctgttccag
       61 ggtccgctgc agcaccacca ccaccaccac ccatggacta gtgctagcaa gaatgcagat
      121 tcctatgcga aaaaacctca catcagcgca ttgaatgccc cacaattgga tcaacgctac
      181 aaaaacgagt tcacgattgg tgcggcagta gaaccttatc aactacaaaa tgaaaaagac
      241 gtacaaatgc taaagcgcca cttcaacagc attgttgccg agaacgtaat gaaaccgatc
      301 agcattcaac ctgaggaagg aaaattcaat tttgaacaag cggatcgaat tgtgaagttc
      361 gctaaggcaa atggcatgga tattcgcttc catacactcg tttggcacag ccaagtacct
      421 caatggttct ttcttgacaa ggaaggtaag ccaatggtta atgaatgcga tccagtgaaa
      481 cgtgaacaaa ataaacaact gctgttaaaa cgacttgaaa ctcatattaa aacgatcgtc
      541 gagcggtaca aagatgacat taagtactgg gacgttgtaa atgaggttgt gggggacgac
      601 ggaaaactgc gcaactctcc atggtatcaa atcgccggca tcgattatat taaagtggca
      661 ttccaagcag ctagaaaata tggcggagac aacattaagc tttacatgaa tgattacaat
      721 acagaagtcg aaccgaagcg aaccgctctt tacaatttag tcaaacaact gaaagaagag
      781 ggtgttccga tcgacggcat cggccatcaa tcccacatcc aaatcggctg gccttctgaa
      841 gcagaaatcg agaaaacgat taacatgttc gccgctctcg gtttagacaa ccaaatcact
      901 gagcttgatg tgagcatgta cggttggccg ccgcgcgctt acccgacgta tgacgccatt
      961 ccaaaacaaa agtttttgga tcaggcagcg cgctatgatc gtttgttcaa actgtatgaa
     1021 aagttgagcg ataaaattag caacgtcacc ttctggggca tcgccgacaa tcatacgtgg
     1081 ctcgacagcc gtgcggatgt gtactatgac gccaacggga atgttgtggt tgacccgaac
     1141 gctccgtacg caaaagtgga aaaagggaaa ggaaaagatg cgccgttcgt ttttggaccg
     1201 gattacaaag tcaaacccgc atattgggct attatcgacc acaaagtggt acctggtact
     1261 ccttctacta aattatacgg cgacgtcaat gatgacggaa aagttaactc aactgacgct
     1321 gtagcattga agagatatgt tttgagatca ggtataagca tcaacactga caatgccgat
     1381 ttgaatgaag acggcagagt taattcaact gacttaggaa ttttgaagag atatattctc
     1441 aaagaaatag atacattgcc gtacaagaac taa
//
