LOCUS       sequence-90787-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  TITLE     SGC Q3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: GRLF1
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..42
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             55..75
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             816..833
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 actttaagaa ggagatatac catgcatcat catcatcatc acagcagcgg cagagaaaac
       61 ttgtatttcc agggcaccta taatatcagc gttgtcggtc tgagcggtac ggaaaaggag
      121 aaagggcaat gcggcattgg taaaagttgt ctgtgcaacc gttttgtacg cccctctgcc
      181 gacgagttcc atctggatca cacttcggtc ctgtcgacaa gcgactttgg gggccgtgtt
      241 gtcaataacg atcatttcct gtactggggt gaggtgtcac gcagtctgga ggactgtgtg
      301 gaatgcaaga tgcacatcgt tgagcagacc gaatttattg atgatcaaac gttccagccg
      361 catcgttcaa ctgcactgca gccgtatatc aaacgcgctg cggccacaaa actggcatcc
      421 gctgagaagc tgatgtactt ttgtaccgac caactgggcc tggaacagga tttcgaacaa
      481 aaacagatgc cggacggtaa actgctggtc gatgggtttc tgctgggcat tgacgtgagt
      541 cggggtatga atcgcaactt cgatgatcag ctgaagtttg taagcaatct gtataaccaa
      601 ctggcgaaaa cgaaaaagcc gatcgttgtg gtcctgacca aatgcgacga gggcgttgaa
      661 cgttacattc gcgatgccca cacttttgca ctgtctaaaa agaatctgca ggtagtggag
      721 acaagtgctc gttcaaacgt caatgttgac ctggcgttct ccacgctggt gcaactgatc
      781 gataaatgat gacgaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc
      841 cggctgctaa caaagcccga angaagctga gttggctgct gccaccgctg agcaataact
//
