LOCUS       sequence-90835-e         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIc F 5-6
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(87..93,94..111,112..117))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(154..278)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(475..492)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
       61 cgacggatcc taactcaaaa tccacacatt atacgagccg gaagcataaa gtgtaaagcc
      121 tggggtgcct aatgcggccc ctcgaggggc cgccatagtg actggatatg ttgtgtttta
      181 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
      241 ttttacgttt ctcgttcagc ttttttgtac aaacttgtgg tctcaccgcc tcattgtctt
      301 cggggtagcg gccgcttgga tcccagaatt ctaggtacct cttaattaat ggagcacaag
      361 actggcctca tgggccttcc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
      421 gctgcattaa catggtcata gctgtttcct tgcgtattgg gcgctctccg cttcctcgct
      481 cactgactcg ctgcgctcgg tcgttcgggt aaagcctggg gtgcctaatg agcaaaaggc
      541 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      601 ccccctgacg agca
//
