LOCUS       sequence-90838-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIc F 3-4
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     103..120
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    320..444
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(485..790)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(587..606)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
       61 tgctcattag gcaccccagg ctttacccga acgaccgagc gcagcgagtc agtgagcgag
      121 gaagcggaga gcgcccaata cgcaaggaaa cagctatgac catgttaatg cagctggcac
      181 gacaggtttc ccgactggaa agcgggcagt gagcggaagg cccatgaggc cagtcttgtg
      241 ctccattaat taagaggtac ctagaattct gggatccaag cggccgctac cccgaagaca
      301 agacagacat gagaccccaa ccactttgta caagaaagct gaacgagaaa cgtaaaatga
      361 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
      421 aacacaacat atccagtcac tatggtcgac ctgcagactg gctgtgtata agggagcctg
      481 acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc
      541 tgagatcagc cacttcttcc ccgataacgg aaaccggcac actggccata tcggtggtca
      601 tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg gggactttat
      661 ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccgggc gtgtcaataa
      721 tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct
      781 taaactgcat ttcaccagcc cctgttctcg tcagcaaaag agccgttcat ttcaataaac
      841 cgggcga
//
