LOCUS       sequence-90843-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for human GTPBP4 with n-terminal 3xFLAG
            epitope fused..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Li S, Wang L, Fu B, Berman MA, Diallo A, Dorf ME
  TITLE     TRIM65 regulates microRNA activity by ubiquitination of TNRC6.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 May 13;111(19):6970-5. doi: 
            10.1073/pnas.1322545111. Epub 2014 Apr 28.
  PUBMED    24778252
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1322545111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-05-13- 13"; volume: "111"; issue: "19"; pages: "6970-5"
COMMENT     Sequence Label: pCMV3FLAG_hGTPBP4
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        32..50
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             94..165
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 tcagatccgc tagcgattac gccaagctcg aaattaaccc tcactaaagg gaacaaaagc
       61 tggagctcca ccgcggtggc ggccgccacc atggattaca aggatgacga cgataaggac
      121 tataaggacg atgatgacaa ggactacaaa gatgatgacg ataaagcccg ggcgggatcc
      181 cccgggctgc aggaattcga tatcgcacat tacaacttca agaaaattac ggtggtgccg
      241 tccgccaagg acttcataga cctcacgttg tcgaagactc aacgaaagac tccaaccgtt
      301 attcataaac attaccaaat acatcgcatt agacattttt acatgagaaa agtcaaattt
      361 actcaacaga attaccatga tagactttca caaattctaa cagatttccc caaattggat
      421 gatattcatc cgttctatgc tgatttgatg aatattctct acgacaagga tcattacaag
      481 ttggctctgg ggcaaataaa tattgccaaa aatttagtgg acaatgttgc taaagattat
      541 gtgcgactga tgaagtatgg cgactctctc taccgctgca aacagctgaa gcgtgcggcc
      601 ctgggacgga tgtgcacagt gatcaagagg cagaagcaga gtttggagta tttggagcaa
      661 gtgcgtcagc atttatcccg tttgccaacc attgatccga ataccaggac cctgcttttg
      721 tgtgggtacc caaatgttgg gaagtccagc ttcgtcaaca aggtgacgag agcagacgtg
      781 gatgtccagc cctatgcgtt cacaaccaag tctctgtttg ttgggcacat ggattataag
      841 tatctacgtt ggcaggttgt agacactcct
//
