LOCUS       sequence-91104-e        4949 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of E gene which produces a lysis protein that 
            induces autolysis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Tanouchi Y, Pai A, Buchler NE, You L
  TITLE     Programming stress-induced altruistic death in engineered bacteria.
  JOURNAL   Mol Syst Biol. 2012;8:626. doi: 10.1038/msb.2012.57.
  PUBMED    23169002
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2012"; volume: "8:626. doi"; pages: " 
            10.1038/msb.2012.57"
COMMENT     Sequence Label: pCSaE500
FEATURES             Location/Qualifiers
     source          1..4949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      1501..1587
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1616..1634)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1630..1677
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1848..2639)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2640..2731)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2779..3517)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(3727..4809)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4771..4790
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4810..4887)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     join(4933..4949,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(4933..4949,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atggctcgag gtattctgac catctaagta catgcgttaa ttatcaggcc
      121 cgctattggc gggccttttt tatttgtgca gcaccccggt caaccaacgg gaaaattcac
      181 gcatcgcggg cgtctccggg cgagattgca aacgcgttat ccagtagctt cccaaatcaa
      241 tctgcgttaa aaacggctga acgatacgtt cactgctgag taaatgcgtg aacattctga
      301 ctggcgcaat tgccacaccc attcccgcct gtgcagcttc caacatggtg actgacgaat
      361 caaacaccat cacattgtgc gtcggtgacg gaggcgcctc tccggccgcc tgcatccaaa
      421 gcgcccattc atcccgccga tacgatcgta ataacggaaa tttcaggata tccgcaggcg
      481 tttgaatctg cgaagccaat gttggcgaac acagtggaga catcagcgcg ctacataaat
      541 attgcgcatc ggtatcgtgc caggctcccc caccgtagcg aatggtataa tccagccctt
      601 cggcggcggg atccacgcga ttgttatggg tagaaatatg caaatcaata tgtgggtaac
      661 tgcgcttaaa atcgctcaac agcgggaaaa gacagccgat agcaaaggtt cccactacgc
      721 caattttaag tttttcctgg gtctgtttag tggcaaaacg atccaacatc cccgccatac
      781 gatcgaagga gtcattaagt acaggtaaca gactttcccc ttcagtcgtc aacattaatc
      841 cacgagaacc gcgcacaaaa agctgacaat tcagctgctg ctccagcgat ttgacatgct
      901 ggctgatggc agaatgcgtc acgttgagct caatcgcagc gcgggtaaag ctgagatgtc
      961 tggccgcggc ttcaaaagcc cgcagcgagt taagagggat atagctacgc gtcatcatta
     1021 agcctgttag aaaaacttat atctgctgct aaatttaacc gtttgtcaac acggtgcaaa
     1081 tcaaacacac tgattgcgtc tgacgggccc ggacaccctt ttgcttgaat tcagatacac
     1141 gtttccaaga ggtaccatgg tacgctggac tttgtgggat accctcgctt tcctgctcct
     1201 gttgagttta ttgctgccgt cattgcttat tatgttcatc ccgtcaacat tcaaacggcc
     1261 tgtctcatca tggaaggcgc tgaatttacg gaaaacatta ttaatggcgt cgagcgtccg
     1321 gttaaagccg ctgaattgtt cgcgtttacc ttgcgtgtac gcgcaggaaa cactgacgtt
     1381 cttactgacg cagaagaaaa cgtgcgtcaa aaattacgtg cggaaggagt gaaagcttgg
     1441 atccctgcag gcctcagggc ccgatcgatg cggccgctta attaattaat ctagaggcat
     1501 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1561 gtgaacgctc tcctgagtag gacaaatccg ccgccctaga cctaggctgc tgccaccgct
     1621 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
     1681 aaacctcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta
     1741 aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtca
     1801 tcgtggccgg atcttgcggc ccctcggctt gaacgaattg ttagacatta tttgccgact
     1861 accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag
     1921 gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat gacgggctga
     1981 tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg
     2041 ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcgcca
     2101 gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc
     2161 tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc aacgctatgt
     2221 tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg ctcgaagata
     2281 cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt agctggataa
     2341 cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg gagaatctcg
     2401 ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt
     2461 tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg
     2521 ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag atggcgctcg
     2581 atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc ttccctcata
     2641 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     2701 atatttgaat gtatttagaa aaataaacaa atagctagct cactcggtcg ctacgctccg
     2761 ggcgtgagac tgcggcgggc gctgcggaca catacaaagt tacccacaga ttccgtggat
     2821 aagcagggga ctaacatgtg aggcaaaaca gcagggccgc gccggtggcg tttttccata
     2881 ggctccgccc tcctgccaga gttcacataa acagacgctt ttccggtgca tctgtgggag
     2941 ccgtgaggct caaccatgaa tctgacagta cgggcgaaac ccgacaggac ttaaagatcc
     3001 ccaccgtttc cggcgggtcg ctccctcttg cgctctcctg ttccgaccct gccgtttacc
     3061 ggatacctgt tccgcctttc tcccttacgg gaagtgtggc gctttctcat agctcacaca
     3121 ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg ggctgtaagc aagaactccc
     3181 cgttcagccc gactgctgcg ccttatccgg taactgttca cttgagtcca acccggaaaa
     3241 gcacggtaaa acgccactgg cagcagccat tggtaactgg gagttcgcag aggatttgtt
     3301 tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt ccggctacac tggaaggaca
     3361 gatttggttg ctgtgctctg cgaaagccag ttaccacggt taagcagttc cccaactgac
     3421 ttaaccttcg atcaaaccac ctccccaggt ggttttttcg tttacagggc aaaagattac
     3481 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctactgaac cgctctagat
     3541 ttcagtgcaa tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt
     3601 gtaattctca tgttagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct
     3661 ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg
     3721 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     3781 ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac
     3841 cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa
     3901 gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg
     3961 gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatgt ccgcaccaac
     4021 gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac
     4081 cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga
     4141 catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata
     4201 tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag
     4261 cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc
     4321 atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg
     4381 aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat
     4441 gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac
     4501 gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt
     4561 aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat
     4621 cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc
     4681 cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac
     4741 cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac
     4801 tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg
     4861 aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct
     4921 gcattaggaa attaatacga ctcactata
//
