LOCUS       sequence-91112-e         293 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pUC cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Vieira J, Messing J
  TITLE     Production of single-stranded plasmid DNA.
  JOURNAL   Methods Enzymol. 1987;153:3-11.
  PUBMED    3323803
REFERENCE   2  (bases 1 to 293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "1987"; volume: "153"; pages: "3-11"
COMMENT     Sequence Label: pUC119
FEATURES             Location/Qualifiers
     source          1..293
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(43..59)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(43..59)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(56..78)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    67..83
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(91..97,98..115,116..121))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    136..157
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(274..291)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tctagagtcg acctgcaggc atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt
       61 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      121 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      181 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      241 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgc
//
