LOCUS       sequence-91119-e         388 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pUC cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Vieira J, Messing J
  TITLE     Production of single-stranded plasmid DNA.
  JOURNAL   Methods Enzymol. 1987;153:3-11.
  PUBMED    3323803
REFERENCE   2  (bases 1 to 388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "1987"; volume: "153"; pages: "3-11"
COMMENT     Sequence Label: pUC118
FEATURES             Location/Qualifiers
     source          1..388
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(37..53)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(37..53)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(50..72)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    61..77
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(85..91,92..109,110..115))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    130..151
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(268..285)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aggatccccg ggtaccgagc tcgaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
       61 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
      121 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
      181 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
      241 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
      301 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
      361 ggataacgca ggaaagaaca tgtgagca
//
