LOCUS       sequence-91158-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  TITLE     pET9d-hnRNP-A1
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET9d-hnRNP-A1
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             102..124
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa ctttaagaag
      121 gagatatacc atgtctaagt cagagtctcc taaagagccc gaacagctga ggaagctctt
      181 cattggaggg ttgagctttg aaacaactga tgagagcctg aggagccatt ttgagcaatg
      241 gggaacgctc acggactgtg tggtaatgag agatccaaac accaagcgct ccaggggctt
      301 tgggtttgtc acatatgcca ctgtggagga ggtggatgca gctatgaatg caaggccaca
      361 caaggtggat ggaagagttg tggaaccaaa gagagctgtc tccagagaag attctcaaag
      421 accaggtgcc cacttaactg tgaaaaagat atttgttggt ggcattaaag aagacactga
      481 agaacatcac ctaagagatt attttgaaca gtatggaaaa attgaagtga ttgaaatcat
      541 gactgaccga ggcagtggca agaaaagggg ctttgccttt gtaacctttg acgaccatga
      601 ctccgtggat aagattgtca ttcagaaata ccatactgtg aatggccaca actgtgaagt
      661 tagaaaagcc ctgtcaaagc aagagatggc tagtgcttca tccagccaaa gaggtcgaag
      721 tggttctgga aactttggtg gtggtcgtgg aggtggtttc ggtgggaatg acaacttcgg
      781 tcgtggagga aacttcagtg gtcgtggtgg ctttggtggc agccgtggtg gtggtggata
      841 tggtggcagt gggg
//
