LOCUS       sequence-91202-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Lopez ME, Klein AD, Dimbil UJ, Scott MP
  TITLE     Anatomically defined neuron-based rescue of neurodegenerative 
            Niemann-Pick type C disorder.
  JOURNAL   J Neurosci. 2011 Mar 23;31(12):4367-78. doi: 
            10.1523/JNEUROSCI.5981-10.2011.
  PUBMED    21430138
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.5981-10"; journalName: "J Neurosci"; date: 
            "2011-03-23- 23"; volume: "31"; issue: "12"; pages: "4367-78"
COMMENT     Sequence Label: NPC1 His6 EGFP
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(42..59)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttgctcacca tggtggcgat ggatcccggg cccgcggtac cgtggtggtg gtggtggtga
       61 aaattgagga gccgttctct ctctgtccct ctgtagcgct cgtaagtggt gtgtctttta
      121 gctttattca ccgacggccc tatgtaactg agtaagacgg gaagaaatat tagtccatga
      181 gtggctccga gtaagaccat ggctaagtac atcctgaagt aaaatatctc aaaaatttga
      241 gatttggcaa aggctaacac cacgatccct ccaaatttcg taagtgtgat tccactgaat
      301 acagaactac ccatgtgggc cagtgcctct tccgcccggc tcactcggct tcctttggta
      361 ctcatggtga atgctctcgt tatatggctg cagaactcca cagaaatgcc gcagctcatc
      421 accaagttga ccaaggagac cgcattcaga ctgatgcccc acagccacat gacaccgaac
      481 atgttgacca ggatcatggc tatggtgata cacatgatga ccgcagacca cagctcacag
      541 cccagaacca ccaaggtcac cagaaatatg gagcccagag acacactgag gttaaagatg
      601 gtgtcatcaa taatggtcag gtactgttca tagaagacgt agaacacact gtaagggaat
      661 acgcggtagt cactcccctt agaacgcatg gtttccgtga tgttactggc tattagccga
      721 gctttcttca tggcatcagt atagtcagcg gaggtcttaa gtatggtgtg gtaggtcatg
      781 aagtaagtgg ccccaatgta agtgtcatct cccacaatgt taacagctga accgtaagca
      841 gcatgtcc
//
