LOCUS       sequence-91268-e         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral plasmid expressing mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  TITLE     Tsoulfas Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-mCherry
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..811
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(246..264)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     514..533
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(871..891)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gacaccgact ctagaggatc cgccgccacc atggtgagca agggcgagga
      121 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
      181 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
      241 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
      301 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
      361 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
      421 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
      481 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
      541 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
      601 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
      661 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
      721 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
      781 caccggcggc atggacgagc tgtacaagta agtcgacaat caacctctgg attacaaaat
      841 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
      901 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
      961 gtataaatcc tggttgctgt ctctttatga ggagttgtg
//
