LOCUS       sequence-91314-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces an active HBFD (carotenoid 4-hydroxy-beta-ring 
            4-dehydrogenase) enzyme in E. coli when expression is induced with 
            arabinose.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Cunningham FX Jr, Gantt E
  TITLE     Elucidation of the pathway to astaxanthin in the flowers of Adonis 
            aestivalis.
  JOURNAL   Plant Cell. 2011 Aug;23(8):3055-69. doi: 10.1105/tpc.111.086827. 
            Epub 2011 Aug 23.
  PUBMED    21862704
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.086827"; journalName: "Plant Cell"; date: 
            "2011-08"; volume: "23"; issue: "8"; pages: "3055-69"
COMMENT     Sequence Label: pHBFD1Bad
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(254..265)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 aacagccaag cttcgaattc ccatatggta ccagctgcag atctcgagaa gcttgtcgac
       61 gaattcagat ctgggctaca taaggaataa tccaatctat tactctactg cttcataatg
      121 aagttaatcg ctccttgtga tgctcgttgt agaagtaact ctctgtcact tattgcaatc
      181 cctccaggct cttctggaaa ccaaactcca ggctgcgtac ttccttcaag aattgccata
      241 gcaaacaccg cagttgaagt tcccactaat acagagagac gttcatgact gagtatacca
      301 aaagtattgc gcccatttgc gcactctaaa tcaacccgca ttgcaagaca ttctcctgca
      361 atcccatcaa aaatttgtac agaagggtac acaaaatttg ccaacattcc gattttattt
      421 ctgtctctca gaataccagg aggaaggaga gttgtcatag ctaccatcgc ccaattccag
      481 aagaaaggtg cagtaccgaa tcgagcactc acagttggta ctcctaagat ctcatgacca
      541 cttcttactt ccggcaagtt ccacaaataa acgtctcttt tcccaactcc cttcccgaag
      601 tcaatgttaa gcttccctgt ataaggcttt aattcgactt tttcgccttc actgtaagca
      661 acaacctctt caccaagaag caagaagcta gtaactaacg acgttggacc agcaccacca
      721 gaacccgcgg taaaatagga gaatcttagt ctttgaggtt cagtgttttc atctcttgct
      781 gatcgcacta gctcagctgc tatcacattg ctcactccag ggaaaattcc agcagttgtt
      841 atggcaggaa cgttggcagc cactgcttta tcatgaaaag actttgcttg catggaataa
      901 cttgtattat cacatacatc cacataggcc gtcctggtag atat
//
