LOCUS       sequence-91456-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Vieira J, Messing J
  TITLE     The pUC plasmids, an M13mp7-derived system for insertion mutagenesis
            and sequencing with synthetic universal primers.
  JOURNAL   Gene. 1982 Oct;19(3):259-68.
  PUBMED    6295879
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1982-10"; volume: "19(3)"; pages: "259-68"
COMMENT     Sequence Label: pUC6
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..36
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(40..46,47..64,65..70))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    85..106
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    211..351
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(277..296)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     437..459
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(497..515)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        583..687
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgtttctgt gtgaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
       61 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      121 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
      181 cgcggggaga ggcggtttgc gtattgggcg cctgatgcgg tattttctcc ttacgcatct
      241 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
      301 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
      361 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
      421 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
      481 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
      541 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      601 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      661 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      721 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
      781 aaagatgctg aagatcagtt gggtgcacga gtgg
//
