LOCUS       sequence-91468-e         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Hillion J, Dhara S, Sumter TF, Mukherjee M, Di Cello F, Belton A, 
            Turkson J, Jaganathan S, Cheng L, Ye Z, Jove R, Aplan P, Lin YW, 
            Wertzler K, Reeves R, Elbahlouh O, Kowalski J, Bhattacharya R, Resar
            LM.
  TITLE     The high-mobility group A1a/signal transducer and activator of 
            transcription-3 axis: an achilles heel for hematopoietic 
            malignancies?
  JOURNAL   Cancer Research 68, 10121, December 2008.
  PUBMED    19074878
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Research 68, 10121, December 2008."
COMMENT     Sequence Label: EF.STAT3DN.Ubc.GFP
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      170..179
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(176..178,179..181,182..694)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(221..242)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(482..501)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
       61 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
      121 acgaagcttg ggctgcaggt cgactctaga ggatccccgg gtaccggtcg ccaccatggt
      181 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      241 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      301 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      361 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
      421 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
      481 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
      541 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
      601 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
      661 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
      721 ctaccagcag aacaccccca tcggcgacgg cc
//
