LOCUS       sequence-91497-e        1105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter assay for RECK 3'UTR that has point mutations on
            miR-21 binding site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Jung HM, Phillips BL, Patel RS, Cohen DM, Jakymiw A, Kong WW, Cheng 
            JQ, Chan EK
  TITLE     Keratinization-associated miR-7 and miR-21 regulate tumor suppressor
            reversion-inducing cysteine-rich protein with kazal motifs (RECK) in
            oral cancer.
  JOURNAL   J Biol Chem. 2012 Aug 24;287(35):29261-72. doi: 
            10.1074/jbc.M112.366518. Epub 2012 Jul 2.
  PUBMED    22761427
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.366518"; journalName: "J Biol Chem"; date: 
            "2012-08-24- 24"; volume: "287"; issue: "35"; pages: "29261-72"
COMMENT     Sequence Label: pMiR-RECK-3'UTR-miR-21-mut
FEATURES             Location/Qualifiers
     source          1..1105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    60..107
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     132..151
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg
       61 aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt
      121 tccttccggt attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa
      181 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
      241 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg
      301 cgtcgcttgg tcggtcattt tcttcgaatc tagatgccgc ggccgcttta agtgcccatt
      361 tttcagcaac attttggtat aatacgcata tccctatgta tttcagaggg tacatataat
      421 actgaaaaaa tacttcatgt aaaatctaag ttcatgtgca tacacacaag aagtattaca
      481 tcaaatacga aatttcaaac tgtgaaacac attcaaagta ttcaatttct tagacaacat
      541 gcatacattt taatactcag cttgacactt ttaaatatat aaaccacaat tctttatact
      601 tctatatcaa aaaagaaagt cagacagaac acttatctac attgtgcata tcacaaaaag
      661 caaatgtgtt ttcacgaatg gaaagcactg ggttaacaca catgattgtc attttacaaa
      721 tatggctatt caccttcttc ataataaaat agaaatataa tgtattattt acatgaacta
      781 cacattattt cactttttgg caattatata agtagcagca attggtttaa agattgatgt
      841 ttaaaaactc tacatttaac ttggatgaat accgatgaat atatatctgg ttaatataaa
      901 aattgcataa tgcaagatgc tttgatgcaa tgtcacttct ccctttcaca aaagtcacag
      961 acacaaatcc gtgaccagtg agcctgttgc ttacaacata taattatatg agtgtcattt
     1021 aatcagcttg cttttgcata acacagtagt tggggttctg aattaaattt ctgtaattta
     1081 atgcaaattt gcctgataat ttaca
//
