LOCUS       sequence-91513-e         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bidirectional construct in the pcDNA5/FRT backbone with GFP and 
            mCherry driven by the same CMV promoter along with a Gateway cloning
            site downstream mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Oikonomou P, Goodarzi H, Tavazoie S
  TITLE     Systematic Identification of Regulatory Elements in Conserved 3' 
            UTRs of Human Transcripts.
  JOURNAL   Cell Rep. 2014 Apr 10;7(1):281-92. doi: 
            10.1016/j.celrep.2014.03.001. Epub 2014 Mar 20.
  PUBMED    24656821
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.03.001"; journalName: "Cell Rep"; date: 
            "2014-04-10- 10"; volume: "7"; issue: "1"; pages: "281-92"
COMMENT     Sequence Label: pcDNA5-FRT-GFP-mCherry-3pGW
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(34..40,41..58,59..64))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(89..213)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(523..542)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aaaatcccga cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg
       61 taaagcctgg ggtgcctaat gcggccgcca tagcgactgg atatgttgtg ttttacagta
      121 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
      181 cgtttctcgt tcagcttttt tgtacaaact tgtaccggtg gacaagctta tcgatgcggc
      241 cgccttactt gtacagctcg tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt
      301 actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccaacttg atgttgacgt
      361 tgtaggcgcc gggcagctgc acgggcttct tggccttgta ggtggtcttg acctcagcgt
      421 cgtagtggcc gccgtccttc agcttcagcc tctgcttgat ctcgcccttc agggcgccgt
      481 cctcggggta cattctctcg gaggaggcct cccagcccat ggtcttcttc tgcattacgg
      541 ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct
      601 gcagggagga gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct
      661 cccac
//
