LOCUS       sequence-91549-e        1045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a 
            C-terminal GFP his tagged protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Parker JL, Newstead S
  TITLE     Molecular basis of nitrate uptake by the plant nitrate transporter 
            NRT1.1.
  JOURNAL   Nature. 2014 Mar 6;507(7490):68-72. doi: 10.1038/nature13116. Epub 
            2014 Feb 26.
  PUBMED    24572366
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13116"; journalName: "Nature"; date: "2014-03-6- 6"; 
            volume: "507"; issue: "7490"; pages: "68-72"
COMMENT     Sequence Label: pDDGFP2_leu2d_NRT1.1
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(12..201)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     213..231
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     249..265
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     250..266
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(296..319)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(320..1030)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
ORIGIN
        1 cgcaattaag ccttcgagcg tcccaaaacc ttctcaagca aggttttcag tataatgtta
       61 catgcgtaca cgcgtctgta cagaaaaaaa agaaaaattt gaaatataaa taacgttctt
      121 aatactaaca taactataaa aaaataaata gggacctaga cttcaggttg tctaactcct
      181 tccttttcgg ttagagcgga tgtgggggga gggcgtgaat gtaagcgtga cataactaat
      241 tacatgactc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agttaatgat
      301 gatgatgatg gtggtggtgt ttgtacaatt catccatacc atgggtaata ccagcagcag
      361 taacaaattc taacaagacc atgtggtctc tcttttcgtt tggatctttg gataaggcag
      421 attgagtgga taagtaatgg ttgtctggta acaagactgg accatcacca attggagtat
      481 tttgttgata atggtcagct aattgaacag aaccatcttc aatgttgtgt ctaattttga
      541 agttaacttt gataccattc ttttgtttgt cagccatgat gtaaacattg tgagagttat
      601 agttgtattc caatttgtga cctaaaatgt taccatcttc tttaaaatca atacctttta
      661 attcgattct attaactaag gtatcacctt caaacttgac ttcagctctg gtcttgtagt
      721 taccgtcatc tttgaaaaaa atagttcttt cttgaacata accttctggc atggcagact
      781 tgaaaaagtc atgttgtttc atatgatctg ggtatctcgc aaaacattga acaccataac
      841 cgaaagtagt gactaaggtt ggccatggaa ctggcaattt accagtagta caaataaatt
      901 ttaaggtcaa tttaccgtaa gtagcgtcac cttcaccttc accggagaca gaaaatttgt
      961 gaccattaac atcaccatct aattcaacca aaattgggac aacgccagtg aataattctt
     1021 cacctttaga aaattgacct tgaaa
//
