LOCUS       sequence-91589-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  TITLE     TLR Signaling Plasmids
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-Myd88-CFP
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(23..736,737..739,740..742))
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(68..89)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     417..436
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     676..697
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctatagaata gggccctcta gattacttgt acagctcgtc catgccgaga gtgatcccgg
       61 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
      121 gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
      181 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
      241 tcttgaagtg ggccttgatg ccgttcttct gcttgtcggc ggtgatatag acgttgtggc
      301 tgatgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
      361 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
      421 tgtagttgcc gtcgtccttg aagaagatgg tacgctcctg gacgtagcct tcgggcatgg
      481 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
      541 cccaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
      601 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
      661 acctgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
      721 gctcctcgcc cttgctcacc atctcgacgg gcagggacaa ggccttggca aggcgagtcc
      781 agaaccaaga tttggtgcag gggttggtgt agtcgcagac agtgatgaac ctcaggatgc
      841 tggggaactc tttcttcatt gccttgtact tgatggggat cagtcgcttc tgatgggcac
      901 ctggagaga
//
