LOCUS       sequence-91621-e        9232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning vector for FAK fret sensor with 30 nm helix.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Ritt M, Guan JL, Sivaramakrishnan S
  TITLE     Visualizing and manipulating focal adhesion kinase regulation in 
            live cells.
  JOURNAL   J Biol Chem. 2013 Mar 29;288(13):8875-86. doi: 
            10.1074/jbc.M112.421164. Epub 2013 Feb 7.
  PUBMED    23393139
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.421164"; journalName: "J Biol Chem"; date: 
            "2013-03-29- 29"; volume: "288"; issue: "13"; pages: "8875-86"
COMMENT     Sequence Label: pCS2-FAK30
FEATURES             Location/Qualifiers
     source          1..9232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             37..60
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(79..81,82..792)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(79..81,82..792)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             2113..2133
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(2788..2790,2791..3501)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5528..5548)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5528..5546)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5567..5583)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5567..5583)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5580..5602)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5591..5607
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5615..5621,5622..5639,5640..5645))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5660..5681
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5798..5815)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5969..6557)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6049..6068)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6728..7519,7520..7588))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7351..7370
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7589..7693)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7719..8174)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7856..7877)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8068..8087
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8248..9232
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttatggact acaaggacga tgacgacaag
       61 ggaagcggag gaagcggagt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      241 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgatgtgctt cgcccgctac
      301 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      481 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
      541 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
      601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      661 ctgcccgaca accactacct gagctaccag tccaaactga gcaaagaccc caacgagaag
      721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      781 gagctgtaca agtctagagg aagcggagga agcggaggaa gcggaggaag cggaggaagc
      841 ggaatggcag cagcttacct tgatccaaac ttgaatcata caccaagttc aagtgcaaag
      901 acgcacctcg gtactgggat ggagcgttcc ccgggggcca tggagcgagt cctaaaggtt
      961 tttcactact ttgaaaacag cagcgagcca acgacgtggg ccagcattat ccggcatgga
     1021 gatgctactg atgttcgagg cataatacag aagattgtgg actgtcacaa agtgaaaaat
     1081 gtggcctgct atgggttgcg actcagtcat ctgcagtctg aggaggttca ctggctgcac
     1141 ctggacatgg gggtatccaa tgtgagagag aaatttgaac tagcacatcc tccagaagaa
     1201 tggaaatatg aactgagaat tcggtacctg cccaaaggat ttctaaacca gttcactgag
     1261 gacaaaccaa ctttaaattt tttctatcag caggtgaaaa atgactatat gttagaaata
     1321 gcagatcaag tggaccagga aattgctttg aaactaggtt gccttgaaat caggagatcc
     1381 tacggagaga tgagaggcaa tgcattagag aagaaatcca actatgaagt gctagaaaaa
     1441 gatgtcggtt taagacgatt ttttccgaag agtttgctag attcagtgaa ggccaaaaca
     1501 ctacgaaaat taatccaaca gacatttcga caatttgcca acctcaacag agaagaaagt
     1561 attttgaaat tctttgagat cctctctcca gtgtacagat ttgacaagga atgcttcaag
     1621 tgtgcccttg gttcaagctg gattatttca gtggagctgg caattggccc agaggaagga
     1681 atcagctacc ttacagacaa gggtgcaaat ccaactcacc tggcagattt taatcaagta
     1741 caaactattc agtattcaaa cagtgaagac aaggacagaa aagggatgtt gcaactgaag
     1801 atagctggtg cacctgagcc tctgacagtg acagcaccat ccttaaccat tgcagagaat
     1861 atggctgact tgatagacgg atactgccga ctggtgaatg gagccacgca atcttttatt
     1921 atcaggccac agaaagaagg tgaaagagct ttaccatcaa taccaaagct ggccaacaat
     1981 gagaagcaag gagtaaggtc gcacacagtc tctgtatcag aaacagatga ctatgcagag
     2041 ataatagatg aagaagatac ttatacaatg ggaagcggag gaagcggaga attcggaagc
     2101 ggaggaagcg gagaaaacct gtattttcag ggcggaagcg gagaagagga agagaagaag
     2161 aaagaagagg aagaaaagaa acaaaaagaa gaacaagaaa gacttgcaaa agaagaggca
     2221 gagagaaaac aaaaagaaga acaagaaaga cttgcaaaag aagaggcaga gagaaaacaa
     2281 aaggaggaag aagagagaaa acaaaaggaa gaagaagaga gaaaacaaaa ggaggaagaa
     2341 gaaagaaaat taaaggagga acaagaaaga aaagctgcag aagaaaagaa agctaaagaa
     2401 gaagctgaga gaaaggctaa agaagaacaa gaaaggaaag ctgaagaaga gagaaagaag
     2461 aaagaagagg aagaaagact tgaaagagaa agaaaagaga gagaagaaca agaaaagaaa
     2521 gccaaagaag aggcagagag aattgcaaag ttagaggctg aaaagaaggc agaagaagaa
     2581 agaaaagcca aagaagaaga agagagaaaa gccaaagaag aagaggaaag aaagaagaaa
     2641 gaggagcaag aaagacttgc aaaagaaaag gaagaagcag aaagaaaagc tgcagaggaa
     2701 aagaaagcta aagaagaaca agaaagaaaa gaaaaggaag aagcagaaag aaaacaaaga
     2761 ggctctggcg gctctggcgg cgcgcccgtg agcaagggcg aggagctgtt caccggggtg
     2821 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     2881 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     2941 aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc
     3001 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     3061 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     3121 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     3181 gaggacggca acatcctggg gcacaagctg gagtacaacg ccatcagcga caacgtctat
     3241 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
     3301 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     3361 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc
     3421 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     3481 ggcatggacg agctgtacaa gttaattaag ggaagcggag gaagcggagg aagcggagga
     3541 agcggaccat caaccagaga ttatgaaatt caaagggaga gaattgaact ggggcgctgc
     3601 attggtgaag gacagtttgg agatgtgcac caaggaattt acatgagtcc ggaaaatcca
     3661 gctatggctg tagcaatcaa aacatgtaaa aactgcacct cagacagcgt tagagaaaag
     3721 ttcctacaag aagccttaac aatgcgtcag tttgatcatc ctcacattgt gaagctcatt
     3781 ggagttatta cagaaaaccc agtgtggata atcatggagc tctgtacact tggagagttg
     3841 agatcgtttc tgcaagtaag aaaattcagc ttggacctgg cctccctcat cctctacgct
     3901 taccagctta gcacagcact tgcttaccta gagagcaaaa gatttgtaca tagagatatt
     3961 gctgctagga acgtgctggt atctgccact gactgtgtga aattgggtga ctttggctta
     4021 tcccgataca tggaagacag tacttactat aaggcgtcca aaggaaagtt acctatcaaa
     4081 tggatggctc cagagtcaat caacttccga cggtttacct cagcaagcga tgtgtggatg
     4141 tttggtgtgt gtatgtggga gatcctgatg catggggtaa agcccttcca gggagtgaaa
     4201 aataatgatg ttattggtcg gattgagaac ggtgagcggc tccccatgcc tccgaactgc
     4261 cctcccaccc tctacagcct tatgaccaag tgctgggcat acgaccctag tagacgaccc
     4321 aggtttactg aacttaaagc acaactcagt acaatactgg aggaggagaa gctgcagcaa
     4381 gaggaacgaa tgagaatgga atccaggcga caagtcacag tatcctggga ctcaggagga
     4441 tcagatgaag ctcctcccaa gcccagcagg cctggttacc ccagcccaag gtccagtgaa
     4501 gggttttatc cgagtcctca gcatatggta cagccaaatc actaccaggt atctggctac
     4561 tctggttctc atgggatacc agccatggca ggcagcattt atcctgggca ggcgtctctc
     4621 ttggatcaaa cagattcctg gaaccatcga cctcaggaag tatcagcatg gcagccaaac
     4681 atggaggatt cgggcacttt ggatgtacga ggaatggggc aggttctgcc cacacatctc
     4741 atggaggaga ggttaataag acaacagcaa gagatggaag aagatcaacg ctggcttgag
     4801 aaagaggaac gattcctggt aatgaaacct gatgtgcggc tctccagagg cagcattgaa
     4861 cgggaggacg gaggtctcca gggcccagct ggtaaccagc acatatatca gcctgtgggt
     4921 aaaccagatc atgccgctcc accaaagaag ccccctcgcc ctggagcccc ccacttgggc
     4981 agcctcgcga gcctgaacag ccccgtggac agctacaacg aaggcgtgaa gatcaagcca
     5041 caggaaatca gccctcctcc tacggccaac ctggaccgct ccaatgacaa agtctatgag
     5101 aatgtaaccg ggctggtgaa agctgtcata gagatgtcca gtaaaataca gccagctccg
     5161 ccagaggagt acgtgcccat ggtaaaggag gttggcttgg cgctgagaac cttgctagca
     5221 acagtggatg agtcgctgcc agtgcttcct gcaagcaccc acagagagat tgagatggcc
     5281 cagaaactgc tgaactctga cctggctgag ctcattaaca agatgaagct ggcccagcag
     5341 tacgtcatga ccagcctgca gcaggagtac aagaagcaaa tgctgacggc tgctcacgct
     5401 ctggctgtgg atgccaagaa cttgctggat gtcatcgatc aagccagact gaaaatgatc
     5461 agccagtcca ggccccactg agcggccgcg gcgccaatgc attgggcccg gtacccagct
     5521 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
     5581 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     5641 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     5701 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     5761 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5821 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5881 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5941 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6001 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6061 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6121 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6181 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6241 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6301 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6361 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     6421 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6481 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     6541 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6601 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6661 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     6721 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     6781 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6841 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6901 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6961 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7021 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7081 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7141 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7201 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7261 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7321 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7381 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7441 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7501 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7561 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7621 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7681 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt
     7741 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     7801 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     7861 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     7921 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     7981 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     8041 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     8101 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     8161 gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     8221 gcgggcctct tcgctattac gccagtcgac catagccaat tcaatatggc gtatatggac
     8281 tcatgccaat tcaatatggt ggatctggac ctgtgccaat tcaatatggc gtatatggac
     8341 tcgtgccaat tcaatatggt ggatctggac cccagccaat tcaatatggc ggacttggca
     8401 ccatgccaat tcaatatggc ggacttggca ctgtgccaac tggggagggg tctacttggc
     8461 acggtgccaa gtttgaggag gggtcttggc cctgtgccaa gtccgccata ttgaattggc
     8521 atggtgccaa taatggcggc catattggct atatgccagg atcaatatat aggcaatatc
     8581 caatatggcc ctatgccaat atggctattg gccaggttca atactatgta ttggccctat
     8641 gccatatagt attccatata tgggttttcc tattgacgta gatagcccct cccaatgggc
     8701 ggtcccatat accatatatg gggcttccta ataccgccca tagccactcc cccattgacg
     8761 tcaatggtct ctatatatgg tctttcctat tgacgtcata tgggcggtcc tattgacgta
     8821 tatggcgcct cccccattga cgtcaattac ggtaaatggc ccgcctggct caatgcccat
     8881 tgacgtcaat aggaccaccc accattgacg tcaatgggat ggctcattgc ccattcatat
     8941 ccgttctcac gccccctatt gacgtcaatg acggtaaatg gcccacttgg cagtacatca
     9001 atatctatta atagtaactt ggcaagtaca ttactattgg aaggacgcca gggtacattg
     9061 gcagtactcc cattgacgtc aatggcggta aatggcccgc gatggctgcc aagtacatcc
     9121 ccattgacgt caatggggag gggcaatgac gcaaatgggc gttccattga cgtaaatggg
     9181 cggtaggcgt gcctaatggg aggtctatat aagcaatgct cgtttaggga ac
//
