LOCUS       sequence-91673-e         525 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of human PITP alpha N90F.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Tilley SJ, Skippen A, Murray-Rust J, Swigart PM, Stewart A, Morgan 
            CP, Cockcroft S, McDonald NQ
  TITLE     Structure-function analysis of human [corrected] 
            phosphatidylinositol transfer protein alpha bound to 
            phosphatidylinositol.
  JOURNAL   Structure. 2004 Feb;12(2):317-26.
  PUBMED    14962392
REFERENCE   2  (bases 1 to 525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Structure."; date: "2004-02"; volume: "12(2)"; pages: "317-26"
COMMENT     Sequence Label: pET14b-PITPalpha N90F
FEATURES             Location/Qualifiers
     source          1..525
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..28
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             38..55
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gggcagcagc catcatcatc atcatcacag cagcggcctg gtgccgcgcg gcagccatat
       61 ggtgctgctc aaggagtatc gagtaatcct gcctgtgtct gtagatgagt atcaagtggg
      121 gcagctgtat tctgtggctg aggccagtaa aaatgaaacg ggtggtggcg aaggcgtgga
      181 ggtcctggtg aatgagccct acgagaagga cggtgagaaa ggccagtaca cacacaagat
      241 ctaccacctg cagagcaaag tacccacgtt tgttcgaatg ctggccccag agggagccct
      301 gaatatacac gagaaagcct ggtttgctta cccctactgc agaaccgtta ttacaaatga
      361 gtacatgaaa gaagactttc tgattaaaat tgaaacctgg cacaaaccag atcttggcac
      421 gcaggagaat gtgcataagc tggagcctga ggcgtggaaa cacgtggaag ccgtatatat
      481 agacattgca gatcgaagcc aagtgctcag caaggattac aaggc
//
