LOCUS       sequence-91683-e        5931 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Forced expression of human FOXH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Takahashi K, Tanabe K, Ohnuki M, Narita M, Sasaki A, Yamamoto M, 
            Nakamura M, Sutou K, Osafune K, Yamanaka S
  TITLE     Induction of pluripotency in human somatic cells via a transient 
            state resembling primitive streak-like mesendoderm.
  JOURNAL   Nat Commun. 2014 Apr 24;5:3678. doi: 10.1038/ncomms4678.
  PUBMED    24759836
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-04-24"; volume: "5:3678. doi"; pages: " 
            10.1038/ncomms4678"
COMMENT     Sequence Label: pMXs-hFOXH1
FEATURES             Location/Qualifiers
     source          1..5931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1945..1969
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3095..3119)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             3436..3905
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4035..4052)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4206..4794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4286..4305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4958..5749,5750..5818))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5581..5600
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5819..5923)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggacctaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatcactg attttatccg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttac ctcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 cccagtgtgg tggtacggga aatcacaagt ttgtacaaaa aagcagaagg gccgtcaagg
     1981 cccaccatgg ggccctgcag cggctcccgc ctggggcccc cagaggcaga gtcgccctcc
     2041 cagcccccta agaggaggaa gaagaggtac ctgcgacatg acaagccccc ctacacctac
     2101 ttggccatga tcgccttggt gattcaggcc gctccctccc gcagactgaa gctggcccag
     2161 atcatccgtc aggtccaggc cgtgttcccc ttcttcaggg aagactacga gggctggaaa
     2221 gactccattc gccacaacct ttcctccaac cgatgcttcc gcaaggtgcc caaggaccct
     2281 gcaaagcccc aggccaaggg caacttctgg gcggtcgacg tgagcctgat cccagctgag
     2341 gcgctccggc tgcagaacac cgccctgtgc cggcgctggc agaacggagg tgcgcgtgga
     2401 gccttcgcca aggacctggg cccctacgtg ctgcacggcc ggccataccg gccgcccagt
     2461 cccccgccac cacccagtga gggcttcagc atcaagtccc tgctaggagg gtccggggag
     2521 ggggcaccct ggccggggct agctccacag agcagcccag ttcctgcagg cacagggaac
     2581 agtggggagg aggcggtgcc caccccaccc cttccctctt ctgagaggcc tctgtggccc
     2641 ctctgccccc ttcctggccc cacgagagtg gagggggaga ctgtgcaggg gggagccatc
     2701 gggccctcaa ccctctcccc agagcctagg gcctggcctc tccacttact gcagggcacc
     2761 gcagttcctg ggggacggtc cagcggggga cacagggcct ccctctgggg gcagctgccc
     2821 acctcctact tgcctatcta cactcccaat gtggtaatgc ccttggcacc accacccacc
     2881 tcctgtcccc agtgtccgtc aaccagccct gcctactggg gggtggcccc tgaaacccga
     2941 gggcccccag ggctgctctg cgatctagac gccctcttcc aaggggtgcc acccaacaaa
     3001 agcatctacg acgtttgggt cagccaccct cgggacctgg cggcccctgg cccaggctgg
     3061 ctgctctcct ggtgcagcct gtgaggcctc atgggcccag ctttcttgta caaagtggtg
     3121 atattccagc tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataataa
     3181 taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta
     3241 aactcgagcg gccgccagca cagtggtcga cgataaaata aaagatttta tttagtctcc
     3301 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     3361 cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag
     3421 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     3481 ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
     3541 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc
     3601 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
     3661 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
     3721 ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg
     3781 actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
     3841 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
     3901 tttcacatgc agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa
     3961 tggccatagt tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     4021 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     4081 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     4141 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4201 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4261 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4321 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4381 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4441 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4501 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4561 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4621 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4681 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4741 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4801 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4861 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttgcggcc ggccgcaaat
     4921 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     4981 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     5041 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     5101 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     5161 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     5221 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     5281 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     5341 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     5401 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     5461 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     5521 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     5581 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     5641 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     5701 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     5761 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     5821 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     5881 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt c
//
