LOCUS       sequence-91863-e        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human TRIM65 mammalian expression vector with 3xFLAG epitope at 
            c-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Li S, Wang L, Fu B, Berman MA, Diallo A, Dorf ME
  TITLE     TRIM65 regulates microRNA activity by ubiquitination of TNRC6.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 May 13;111(19):6970-5. doi: 
            10.1073/pnas.1322545111. Epub 2014 Apr 28.
  PUBMED    24778252
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1322545111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-05-13- 13"; volume: "111"; issue: "19"; pages: "6970-5"
COMMENT     Sequence Label: pCMVhTRIM65_3Flag
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..28
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9..27
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(81..152)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 gtgaattgta atacgactca ctatagggcg aattgggtac acttacctgg taccttaatt
       61 aattaaggta ccgggcccta tttatcgtca tcatctttgt agtccttgtc atcatcgtcc
      121 ttatagtcct tatcgtcgtc atccttgtaa tcctcgacat cgatgctgag cacctcttcc
      181 tggggccccg gagggaacac agcccctggc tgatggcaca gggtcagggt cctaccctcg
      241 aggagccaga agacgggggt gaggggctgg ttgaagaggg catggaaggt gtacaggggc
      301 tgggtctggg gctccaggct gtagaaggtg aggcagcctg aggccaggtc caaatccatg
      361 cccaggagcc gccctgacac ccctgggagg cgctgggctt ccccgttgtg ccaggcctgg
      421 aggctgtcct cctggacgca gagcccccag gagcagggtc cccggccaat gttgtctgtg
      481 tggggcccca gcctgcaccg tggcagttgc gggtaggaga cgcccagtgt caccgagtgg
      541 tctgacgcgc gcacctccca gtagtggtgc ccggcctgga agctctgggc acattgcacc
      601 tgccagagct caaagctgcc gggcccgcct gggccccggg actgacgaca gtgcttcacc
      661 tgctggtcct ggcgcgacag atagaagtga cggttggcgc tgactggatc aaaggtcaga
      721 ttgcgataat tctgccagag tttcctcctc agtggacaaa ctgtgcttgg gaccggtgcc
      781 aggggacctg gggcctccac gggggctaag tccacaggct tggctggtgc cccagggtgg
      841 ctcccctctt ccaagaggag gccacacagc cggcttagca actgcttcag gtcacccagc
      901 tgttggtctt catcccactg cagaggggtc agtggcccaa gaggccctgg gggctggagg
      961 agctgcgatt cctgcaggaa ggtctgctca tccacctgct ccaggagctc ccggatcctg
     1021 cagccatggc gagccacagc ctccaagtgg ccccgcagcc gctgctcctc gtctcga
//
