LOCUS       sequence-91892-e         610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flag-CHES1L mutant lacking the forkhead binding site and N-terminal 
            domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Huot G, Vernier M, Bourdeau V, Doucet L, Saint-Germain E, 
            Gaumont-Leclerc MF, Moro A, Ferbeyre G
  TITLE     CHES1/FOXN3 regulates cell proliferation by repressing PIM2 and 
            protein biosynthesis.
  JOURNAL   Mol Biol Cell. 2014 Mar;25(5):554-65. doi: 10.1091/mbc.E13-02-0110. 
            Epub 2014 Jan 8.
  PUBMED    24403608
REFERENCE   2  (bases 1 to 610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-02-0110"; journalName: "Mol Biol Cell"; date: 
            "2014-03"; volume: "25"; issue: "5"; pages: "554-65"
COMMENT     Sequence Label: pLPC-Flag-CHES1L-dFN
FEATURES             Location/Qualifiers
     source          1..610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             135..158
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc
      121 tggaattcgc catggactac aaagacgatg acgacaaggg cacaccttat cacccacacc
      181 cacacgtgtt caatacacct cccacctgtc ctcaggcata tcaaagcaca tcaggtccac
      241 ccatctggcc gggcagtacc ttcttcaaga gaaatggagc ccttctccaa gatcctgaca
      301 ttgatgctgc cagtgccatg atgcttttga atactccccc tgagatacaa gcaggttttc
      361 ctccaggagt gatccaaaat ggagcgcggg tcctgagccg agggctgttt cctggcgtgc
      421 ggccgctgcc aatcactccc attggggtga cagcggccat gaggaatggc atcaccagct
      481 gccggatgcg gactgagagt gagccatctt gtggctcccc agtggtcagc ggagacccca
      541 aggaggatca caactacagc agtgccaagt cctccaacgc ccggagcacc tcgcccacca
      601 gcgactccat
//
