LOCUS       sequence-91893-e         484 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flag-CHES1L mutant lacking the N-terminal domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Huot G, Vernier M, Bourdeau V, Doucet L, Saint-Germain E, 
            Gaumont-Leclerc MF, Moro A, Ferbeyre G
  TITLE     CHES1/FOXN3 regulates cell proliferation by repressing PIM2 and 
            protein biosynthesis.
  JOURNAL   Mol Biol Cell. 2014 Mar;25(5):554-65. doi: 10.1091/mbc.E13-02-0110. 
            Epub 2014 Jan 8.
  PUBMED    24403608
REFERENCE   2  (bases 1 to 484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-02-0110"; journalName: "Mol Biol Cell"; date: 
            "2014-03"; volume: "25"; issue: "5"; pages: "554-65"
COMMENT     Sequence Label: pLPC-Flag-CHES1L-dN
FEATURES             Location/Qualifiers
     source          1..484
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..57
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             125..148
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
       61 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc
      121 catggactac aaagacgatg acgacaaggg caaccccaac tgcaaacccc cctactcctt
      181 cagctgcctc atatttatgg ccatcgagga ctctccaacc aagcgcctgc cagtgaagga
      241 tatctacaac tggatcttgg aacattttcc gtattttgca aatgcaccta ctgggtggaa
      301 aaactcagtg agacacaatt tatcattgaa taagtgtttt aagaaagtgg acaaagagag
      361 gagtcagagt attgggaaag ggtcgttgtg gtgcatagac ccagagtata gacaaaatct
      421 aattcaggct ttgaaaaaga caccttatca cccacaccca cacgtgttca atacacctcc
      481 cacc
//
