LOCUS       sequence-91978-e        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses beta-glucosidase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Carlin DA, Caster RW, Wang X, Betzenderfer SA, Chen CX, Duong VM, 
            Ryklansky CV, Alpekin A, Beaumont N, Kapoor H, Kim N, Mohabbot H, 
            Pang B, Teel R, Whithaus L, Tagkopoulos I, Siegel JB
  TITLE     Kinetic Characterization of 100 Glycoside Hydrolase Mutants Enables 
            the Discovery of Structural Features Correlated with Kinetic 
            Constants.
  JOURNAL   PLoS One. 2016 Jan 27;11(1):e0147596. doi: 
            10.1371/journal.pone.0147596. eCollection 2016.
  PUBMED    26815142
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-27"; volume: "11(1):e0147596. doi"; pages: " 
            10.1371/journal.pone.0147596. eCollection 2016"
COMMENT     Sequence Label: BglB in pET29b(+)
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     74..93
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        74..92
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    93..117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             132..154
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatcgatctc
       61 gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct
      121 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgagcgag aacaccttta
      181 tctttccggc aacctttatg tggggcacca gcaccagcag ctatcagatt gaaggtggca
      241 ccgatgaagg tggtcgtacc ccgagcattt gggatacctt ttgtcagatt ccgggtaaag
      301 ttattggtgg tgattgtggt gatgttgcct gtgatcattt tcaccacttt aaagaagatg
      361 tgcagctgat gaaacagctg ggttttctgc attatcgttt tagcgttgca tggcctcgta
      421 ttatgcctgc agcaggtatt atcaatgaag agggtctgct gttttatgag catctgctgg
      481 atgaaattga actggcaggt ctgattccga tgctgaccct gtatcattgg gatctgccgc
      541 agtggattga agatgaaggc ggttggaccc agcgtgaaac cattcagcat ttcaaaacct
      601 atgccagcgt tatcatggat cgttttggtg aacgtattaa ttggtggaac accatcaatg
      661 aaccgtattg tgcaagcatt ctgggttatg gcaccggtga acatgcaccg ggtcatgaaa
      721 attggcgtga agcatttacc gcagcacatc atattctgat gtgtcatggt attgcaagca
      781 acctgcataa agaaaaaggt ctgaccggta aaattggcat taccctgaat atggaacatg
      841 ttgatgcagc aagcgaacgt ccggaagatg ttgccgcagc aattcgtcgt gatggtttta
      901 tcaatcgttg gtttgcagaa ccgctgttca atggtaaata tcctgaagat atggtggaat
      961 ggtatggcac ctatctgaat ggtctggatt ttgttcagcc tgg
//
