LOCUS       sequence-91981-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Vieira J, Messing J
  TITLE     The pUC plasmids, an M13mp7-derived system for insertion mutagenesis
            and sequencing with synthetic universal primers.
  JOURNAL   Gene. 1982 Oct;19(3):259-68.
  PUBMED    6295879
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1982-10"; volume: "19(3)"; pages: "259-68"
COMMENT     Sequence Label: pUC6
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..30
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14..30
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(46..63)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(46..62)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(55..77)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(217..234)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(468..487)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atacaatttc acacaggaaa cagctatgac catgattacg aattcactgg ccgtcgtttt
       61 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
      121 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
      181 gcgcagcctg aatggcgaat ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      241 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      301 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      361 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      421 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      481 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      541 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      601 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      661 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      721 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      781 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      841 tatct
//
