LOCUS       sequence-91993-e         586 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ratiometric (Blue to Green) fluorescent indicator for calcium 
            signaling in the endoplasmic reticulum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Suzuki J, Kanemaru K, Ishii K, Ohkura M, Okubo Y, Iino M
  TITLE     Imaging intraorganellar Ca(2+) at subcellular resolution using 
            CEPIA.
  JOURNAL   Nat Commun. 2014 Jun 13;5:4153. doi: 10.1038/ncomms5153.
  PUBMED    24923787
REFERENCE   2  (bases 1 to 586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-06-13"; volume: "5:4153. doi"; pages: " 
            10.1038/ncomms5153"
COMMENT     Sequence Label: pCIS GEM-CEPIA1er
FEATURES             Location/Qualifiers
     source          1..586
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(213..242)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
       61 tttggcagag ggaaaaagat ctcagtggta tttgtgagcc agggcattgg ccacaccagc
      121 caccaccttc tgataggcag cctgcacctg aggagtgaag cggccgctct agaactagtc
      181 tacagctcgt ccttctcgct tgcggcccca ttcagatcct cttctgagat gagtttttgt
      241 tctgcggccg ccttcgctgt catcatttgt acaaagtctt cgtagtttac ctgaccttct
      301 ccatcgatgt ctgctaccct gatcatttca tcaacctctt catctgttaa cttctctcca
      361 aggtttgtca tcacgtggcg aagctctgct gcgccgatgt agccattgcc atccttatcc
      421 cacacacgga acgcttcgcg aatttcctct tcactgtctg tgtcctgcat tttaggtgcc
      481 atcattgtca ggaactcagg gaagtcgatt gtgccgtcac cgtcggcatc tacttcattg
      541 atcatgtcct ggagctctgc ttctgtgggg ttctgcccca gagacc
//
