LOCUS       sequence-92003-e        7280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for baculovirus expression of CAMSAP2 CC in Sf9 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Hendershott MC, Vale RD
  TITLE     Regulation of microtubule minus-end dynamics by CAMSAPs and 
            Patronin.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):5860-5. doi: 
            10.1073/pnas.1404133111. Epub 2014 Mar 26.
  PUBMED    24706919
REFERENCE   2  (bases 1 to 7280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1404133111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-04-22- 22"; volume: "111"; issue: "16"; pages: "5860-5"
COMMENT     Sequence Label: pFasBac+GFP-CAMSAP2 CC
FEATURES             Location/Qualifiers
     source          1..7280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     299..320
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        484..588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(589..657,658..1449)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(807..826)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1620..2208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2109..2128
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2362..2379
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2511..2735
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     promoter        complement(join(3506..3511,3512..3528,3529..3534))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3886..3977
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3902..3919
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4044..4061
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4083..4103
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(4116..4118,4119..4829)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4116..4118,4119..4829)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4158..4179)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4419..4438)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4766..4787
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     RBS             5758..5766
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    6770..6904
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6807..6826)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6861..6880
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  6933..7098
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gccccgcatg gatttgactt ggtcagggcc
     3241 gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact
     3301 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
     3361 cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat
     3421 gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg
     3481 tgagcgcata cgctacttgc attacagttt acgaaccgaa caggcttatg tcaactgggt
     3541 tcgtgccttc atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt
     3601 cgaggcattt ctgtcctggc tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca
     3661 ggcattggcg gccttgctgt tcttctacgg caaggtgctg tgcacggatc tgccctggct
     3721 tcaggagatc ggaagacctc ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga
     3781 agtggttcgc atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc aggactctag
     3841 ctatagttct agtggttggc tacgtatact ccggaatatt aatagatcat ggagataatt
     3901 aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa
     3961 taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg cgcggatctc
     4021 ggtccgaaac catgtcgtac taccatcacc atcaccatca cgattacgat atcccaacga
     4081 ccgaaaacct gtattttcag ggcgccatgg atccagtgag caagggcgag gagctgttca
     4141 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     4201 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     4261 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     4321 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     4381 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     4441 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     4501 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     4561 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     4621 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     4681 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     4741 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     4801 tcactctcgg catggacgag ctgtacaagg agctactgaa tcataccgat ggaaaaagta
     4861 gtggaagcag ttctcaaaaa actacaccag aaggctctga acttaatatt cctcatgtgg
     4921 ttgcttgggc acaaattcca gaagaaacag ggcttccaca gggacgggac actacccagc
     4981 tgttggcctc tgaaatggtg catcttagga tgaaactaga agaaaagagg cgtgctatag
     5041 aagcccagaa aaagaaaatg gaagctgctt ttaccaaaca gagacagaaa atgggaagga
     5101 cagcattcct tactgtagtg aaaaagaaag gggatgggat atctcctcta cgagaggaag
     5161 cggcgggtgc agaagatgag aaagtatata ctgatcgagc aaaagaaaag gaatcacaaa
     5221 aaactgatgg acaaaggagc aagtcactgg cagatataaa agagagcatg gagaatcctc
     5281 aagccaaatg gctaaagtct ccaactacac ctattgatcc tgagaagcag tggaacctgg
     5341 caagcccctc agaagaaact ttaaatgaag gagagatttt agaatatacc aaatccattg
     5401 aaaagttaaa ttcatccctg cattttctac aacaagaaat gcaacgcttg tcacttcagc
     5461 aggagatgtt aatgcagatg agagagcaac aatcttgggt gatttcacct ccacaaccct
     5521 ctccacagaa acagattcga gattttaagc cttctaagca ggcaggcctg tcatcagcca
     5581 ttgcaccatt ctcctcagac tcccctcgtc ctactcaccc atctccacag tcttctaaca
     5641 ggaaaagtgc atctttttct gttaaaagtc aaaggactcc taggccaaat gagttaaaaa
     5701 taacaccttt gaatcgaacc ttgacacctc ctcggtctgt ggatagcctt cctcggttaa
     5761 ggaggttttc accaagtcaa gttcctattc aaactaggtc atttgtatgt tttggggatg
     5821 atggagaacc tcagttaaag gaatccaaac ctaaagagga agttaaaaag gaggaattgg
     5881 aatccaaagg gactttggaa cagcgtggac ataatccaga agaaaaggaa atcaaacctt
     5941 ttgagtcaac agtctctgaa gtcctatcac tgcctgtcac agagactgta tgtctgacac
     6001 caaatgagga ccaattgaat caacccacag aaccccctcc taaacccgtt ttcccaccca
     6061 ctgctccaaa aaatgttaat ctgattgaag tttccctctc agatttgaaa ccccctgaaa
     6121 aggctgatgt acctgttgaa aaatatgatg gagaaagtga taaagaacaa tttgatgatg
     6181 accagaaagt atgctgtgga ttctttttta aggatgatca aaaagcagaa aatgatatgg
     6241 caatgaaacg ggcagctttg ttggagaaaa gattaagaag ggaaaaggaa actcagctcc
     6301 ggaaacaaca gttggaagca gaaatggagc ataagaagga ggaaacaagg cgtaaaactg
     6361 aggaagaacg tcagaagaaa gaagatgaga gagcacgcag agaatttatt aggcaagaat
     6421 atatgaggcg gaaacaactg aaactaatgg aagatatgga tacagtaatt aaaccccgtc
     6481 ctcaagtagt aaaacaaaaa aaacagcgac caaaatctat tcacagagat catattgaat
     6541 cccccaaaac accaataaag ggtcctccag tctctagcct ttctttggca tcgctgaaca
     6601 cgggtgataa cgagagtgta tgatcgaggc atgcggtacc aagcttgtcg agaagtacta
     6661 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     6721 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     6781 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6841 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6901 gatctgatca ctgcttgagc ctaggagatc cgaaccagat aagtgaaatc tagttccaaa
     6961 ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat
     7021 tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa
     7081 ctattttgtc cgcccacagc ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc
     7141 tgccaactcc atgtgacaaa ccgtcatctt cggctacttt ttctctgtca cagaatgaaa
     7201 atttttctgt catctcttcg ttattaatgt ttgtaattga ctgaatatca acgcttattt
     7261 gcagcctgaa tggcgaatgg
//
