LOCUS       sequence-92012-e         609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green fluorescent indicator for calcium imaging in the mitochondria 
            (low affinity variant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Suzuki J, Kanemaru K, Ishii K, Ohkura M, Okubo Y, Iino M
  TITLE     Imaging intraorganellar Ca(2+) at subcellular resolution using 
            CEPIA.
  JOURNAL   Nat Commun. 2014 Jun 13;5:4153. doi: 10.1038/ncomms5153.
  PUBMED    24923787
REFERENCE   2  (bases 1 to 609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-06-13"; volume: "5:4153. doi"; pages: " 
            10.1038/ncomms5153"
COMMENT     Sequence Label: pCMV CEPIA4mt
FEATURES             Location/Qualifiers
     source          1..609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..116
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             138..212
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             252..269
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             273..305
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(309..317,318..332)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      339..348
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             360..416
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
ORIGIN
        1 tctggctaac tagagaaccc actgcttact ggcacgtgac catgtccgtc ctgacgccgc
       61 tgctgctgcg gggcttgaca ggctcggccc ggcggctccc agtgccgcgc gccaaaattc
      121 attcactggg ggaccccatg agcgtgctca ccccactcct gctgcggggg ctgaccggca
      181 gcgctaggcg gctgccagtc ccgcgggcca agatccacag tctcggcgat cccggatcca
      241 tgcggggttc tcatcatcat catcatcatg gtatggctag catgactggt ggacagcaaa
      301 tgggtcggga tctgtacgac gatgacgata aggatctcgc caccatggtc gactcatcac
      361 gtcgtaagtg gaataagaca ggtcacgcag tcagagctat aggtcggctg agctcactcg
      421 agaacgtcta tatcatggcc gacaagcaga agaacggcat caaggcgaac ttcaagatcc
      481 gccacaacat cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag aacaccccca
      541 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaaacttt
      601 cgaaagacc
//
