LOCUS       sequence-92024-e       15432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ChR2-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15432)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRVdG-4ChR2-mCherry
FEATURES             Location/Qualifiers
     source          1..15432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4689..4698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4695..5621
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             5634..6341
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(5776..5794)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6044..6063
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             8093..8104
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(13104..13122)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      13118..13165
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(13188..13204)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13188..13204)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(13241..13261)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13241..13259)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13280..13296)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13280..13296)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13293..13315)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13304..13320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13328..13334,13335..13352,13353..13358))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13373..13394
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13511..13528)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13682..14270)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13762..13781)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14441..15232,15233..15301))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15064..15083
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15302..15406)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt gaagcctgag
     1501 attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa aaagccctgt
     1561 ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt gtcaggcatg
     1621 agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc aatgcagttt
     1681 tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc acgaaaagga
     1741 gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga agggaattgg
     1801 gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca tgcgtcctta
     1861 gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa cactggtaac
     1921 tataagacaa acattgcaga caggatagag cagatttttg agacagcccc ttttgttaaa
     1981 atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg gagtactata
     2041 ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat tgagcatcta
     2101 tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc aggactggta
     2161 tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat actatatttc
     2221 ttccacaaga actttgagga agagataaga agaatgtttg agccagggca ggagacagct
     2281 gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa atctccttat
     2341 tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg ctatatgggt
     2401 caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga aatgtctgtt
     2461 ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag aagattcttc
     2521 agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac tgacgtagca
     2581 ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg tgaaaccaga
     2641 agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa gagatctcac
     2701 atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc attcgccgag
     2761 tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa tgccggaaat
     2821 ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg aaccatccca
     2881 aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc cgatcttgag
     2941 atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca ggctcatctc
     3001 caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact tcacctggat
     3061 gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa gtatcgagag
     3121 gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata cctggaaaat
     3181 gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa gatatggtca
     3241 cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc tccaggaaag
     3301 tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga gacaacaccc
     3361 actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca aattgcttct
     3421 ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc agtggaggct
     3481 gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc ctctcgatcc
     3541 tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga tatagttaaa
     3601 gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa actcccccta
     3661 agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt gttagtcgaa
     3721 tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc ttgctaaccg
     3781 aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag tcaacatgaa
     3841 aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga aaaaccgcag
     3901 ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg
     3961 gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa
     4021 cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct
     4081 gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt
     4141 agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg
     4201 ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga
     4261 aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg
     4321 attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa
     4381 catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga
     4441 agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc aaatttatca
     4501 cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg gagcaatata
     4561 acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca agttgattac
     4621 ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca aaagaccccg
     4681 ggaaagccgc caccatggac tatggcggcg ctttgtctgc cgtcggacgc gaacttttgt
     4741 tcgttactaa tcctgtggtg gtgaacgggt ccgtcctggt ccctgaggat caatgttact
     4801 gtgccggatg gattgaatct cgcggcacga acggcgctca gaccgcgtca aatgtcctgc
     4861 agtggcttgc agcaggattc agcattttgc tgctgatgtt ctatgcctac caaacctgga
     4921 aatctacatg cggctgggag gagatctatg tgtgcgccat tgaaatggtt aaggtgattc
     4981 tcgagttctt ttttgagttt aagaatccct ctatgctcta ccttgccaca ggacaccggg
     5041 tgcagtggct gcgctatgca gagtggctgc tcacttgtcc tgtcatcctt atccgcctga
     5101 gcaacctcac cggcctgagc aacgactaca gcaggagaac catgggactc cttgtctcag
     5161 acatcgggac tatcgtgtgg ggggctacca gcgccatggc aaccggctat gttaaagtca
     5221 tcttcttttg tcttggattg tgctatggcg cgaacacatt ttttcacgcc gccaaagcat
     5281 atatcgaggg ttatcatact gtgccaaagg gtcggtgccg ccaggtcgtg accggcatgg
     5341 catggctgtt tttcgtgagc tggggtatgt tcccaattct cttcattttg gggcccgaag
     5401 gttttggcgt cctgagcgtc tatggctcca ccgtaggtca cacgattatt gatctgatga
     5461 gtaaaaattg ttgggggttg ttgggacact acctgcgcgt cctgatccac gagcacatat
     5521 tgattcacgg agatatccgc aaaaccacca aactgaacat cggcggaacg gagatcgagg
     5581 tcgagactct cgtcgaagac gaagccgagg ccggagccgt gccagcggcc gccgtgagca
     5641 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     5701 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     5761 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     5821 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     5881 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     5941 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     6001 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     6061 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     6121 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     6181 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     6241 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     6301 gccgccactc caccggcggc atggacgagc tgtacaagta atgacccggg ttataaagtg
     6361 ctgggtcatc taagcttttc agtcgagaaa aaaacattag atcagaagaa caactggcaa
     6421 cacttctcaa cctgagactt acttcaagat gctcgatcct ggagaggtct atgatgaccc
     6481 tattgaccca atcgagttag aggctgaacc cagaggaacc cccattgtcc ccaacatctt
     6541 gaggaactct gactacaatc tcaactctcc tttgatagaa gatcctgcta gactaatgtt
     6601 agaatggtta aaaacaggga atagacctta tcggatgact ctaacagaca attgctccag
     6661 gtctttcaga gttttgaaag attatttcaa gaaggtagat ttgggttctc tcaaggtggg
     6721 cggaatggct gcacagtcaa tgatttctct ctggttatat ggtgcccact ctgaatccaa
     6781 caggagccgg agatgtataa cagacttggc ccatttctat tccaagtcgt cccccataga
     6841 gaagctgttg aatctcacgc taggaaatag agggctgaga atccccccag agggagtgtt
     6901 aagttgcctt gagagggttg attatgataa tgcatttgga aggtatcttg ccaacacgta
     6961 ttcctcttac ttgttcttcc atgtaatcac cttatacatg aacgccctag actgggatga
     7021 agaaaagacc atcctagcat tatggaaaga tttaacctca gtggacatcg ggaaggactt
     7081 ggtaaagttc aaagaccaaa tatggggact gctgatcgtg acaaaggact ttgtttactc
     7141 ccaaagttcc aattgtcttt ttgacagaaa ctacacactt atgctaaaag atcttttctt
     7201 gtctcgcttc aactccttaa tggtcttgct ctctccccca gagccccgat actcagatga
     7261 cttgatatct caactatgcc agctgtacat tgctggggat caagtcttgt ctatgtgtgg
     7321 aaactccggc tatgaagtca tcaaaatatt ggagccatat gtcgtgaata gtttagtcca
     7381 gagagcagaa aagtttaggc ctctcattca ttccttggga gactttcctg tatttataaa
     7441 agacaaggta agtcaacttg aagagacgtt cggtccctgt gcaagaaggt tctttagggc
     7501 tctggatcaa ttcgacaaca tacatgactt ggtttttgtg tttggctgtt acaggcattg
     7561 ggggcaccca tatatagatt atcgaaaggg tctgtcaaaa ctatatgatc aggttcacct
     7621 taaaaaaatg atagataagt cctaccagga gtgcttagca agcgacctag ccaggaggat
     7681 ccttagatgg ggttttgata agtactccaa gtggtatctg gattcaagat tcctagcccg
     7741 agaccacccc ttgactcctt atatcaaaac ccaaacatgg ccacccaaac atattgtaga
     7801 cttggtgggg gatacatggc acaagctccc gatcacgcag atctttgaga ttcctgaatc
     7861 aatggatccg tcagaaatat tggatgacaa atcacattct ttcaccagaa cgagactagc
     7921 ttcttggctg tcagaaaacc gaggggggcc tgttcctagc gaaaaagtta ttatcacggc
     7981 cctgtctaag ccgcctgtca atccccgaga gtttctgagg tctatagacc tcggaggatt
     8041 gccagatgaa gacttgataa ttggcctcaa gccaaaggaa cgggaattga agattgaagg
     8101 tcgattcttt gctctaatgt catggaatct aagattgtat tttgtcatca ctgaaaaact
     8161 cttggccaac tacatcttgc cactttttga cgcgctgact atgacagaca acctgaacaa
     8221 ggtgtttaaa aagctgatcg acagggtcac cgggcaaggg cttttggact attcaagggt
     8281 cacatatgca tttcacctgg actatgaaaa gtggaacaac catcaaagat tagagtcaac
     8341 agaggatgta ttttctgtcc tagatcaagt gtttggattg aagagagtgt tttctagaac
     8401 acacgagttt tttcaaaagg cctggatcta ttattcagac agatcagacc tcatcgggtt
     8461 acgggaggat caaatatact gcttagatgc gtccaacggc ccaacctgtt ggaatggcca
     8521 ggatggcggg ctagaaggct tacggcagaa gggctggagt ctagtcagct tattgatgat
     8581 agatagagaa tctcaaatca ggaacacaag aaccaaaata ctagctcaag gagacaacca
     8641 ggttttatgt ccgacataca tgttgtcgcc agggctatct caagaggggc tcctctatga
     8701 attggagaga atatcaagga atgcactttc gatatacaga gccgtcgagg aaggggcatc
     8761 taagctaggg ctgatcatca agaaagaaga gaccatgtgt agttatgact tcctcatcta
     8821 tggaaaaacc cctttgttta gaggtaacat attggtgcct gagtccaaaa gatgggccag
     8881 agtctcttgc gtctctaatg accaaatagt caacctcgcc aatataatgt cgacagtgtc
     8941 caccaatgcg ctaacagtgg cacaacactc tcaatctttg atcaaaccga tgagggattt
     9001 tctgctcatg tcagtacagg cagtctttca ctacctgcta tttagcccaa tcttaaaggg
     9061 aagagtttac aagattctga gcgctgaagg ggagagcttt ctcctagcca tgtcaaggat
     9121 aatctatcta gatccttctt tgggagggat atctggaatg tccctcggaa gattccatat
     9181 acgacagttc tcagaccctg tctctgaagg gttatccttc tggagagaga tctggttaag
     9241 ctcccaagag tcctggattc acgcgttgtg tcaagaggct ggaaacccag atcttggaga
     9301 gagaacactc gagagcttca ctcgccttct agaagatccg accaccttaa atatcagagg
     9361 aggggccagt cctaccattc tactcaagga tgcaatcaga aaggctttat atgacgaggt
     9421 ggacaaggtg gaaaattcag agtttcgaga ggcaatcctg ttgtccaaga cccatagaga
     9481 taattttata ctcttcttaa tatctgttga gcctctgttt cctcgatttc tcagtgagct
     9541 attcagttcg tcttttttgg gaatccccga gtcaatcatt ggattgatac aaaactcccg
     9601 aacgataaga aggcagttta gaaagagtct ctcaaaaact ttagaagaat ccttctacaa
     9661 ctcagagatc cacgggatta gtcggatgac ccagacacct cagagggttg ggggggtgtg
     9721 gccttgctct tcagagaggg cagatctact tagggagatc tcttggggaa gaaaagtggt
     9781 aggcacgaca gttcctcacc cttctgagat gttgggatta cttcccaagt cctctatttc
     9841 ttgcacttgt ggagcaacag gaggaggcaa tcctagagtt tctgtatcag tactcccgtc
     9901 ctttgatcag tcattttttt cacgaggccc cctaaaggga tacttgggct cgtccacctc
     9961 tatgtcgacc cagctattcc atgcatggga aaaagtcact aatgttcatg tggtgaagag
    10021 agctctatcg ttaaaagaat ctataaactg gttcattact agagattcca acttggctca
    10081 agctctaatt aggaacatta tgtctctgac aggccctgat ttccctctag aggaggcccc
    10141 tgtcttcaaa aggacggggt cagccttgca taggttcaag tctgccagat acagcgaagg
    10201 agggtattct tctgtctgcc cgaacctcct ctctcatatt tctgttagta cagacaccat
    10261 gtctgatttg acccaagacg ggaagaacta cgatttcatg ttccagccat tgatgcttta
    10321 tgcacagaca tggacatcag agctggtaca gagagacaca aggctaagag actctacgtt
    10381 tcattggcac ctccgatgca acaggtgtgt gagacccatt gacgacgtga ccctggagac
    10441 ctctcagatc ttcgagtttc cggatgtgtc gaaaagaata tccagaatgg tttctggggc
    10501 tgtgcctcac ttccagaggc ttcccgatat ccgtctgaga ccaggagatt ttgaatctct
    10561 aagcggtaga gaaaagtctc accatatcgg atcagctcag gggctcttat actcaatctt
    10621 agtggcaatt cacgactcag gatacaatga tggaaccatc ttccctgtca acatatacgg
    10681 caaggtttcc cctagagact atttgagagg gctcgcaagg ggagtattga taggatcctc
    10741 gatttgcttc ttgacaagaa tgacaaatat caatattaat agacctcttg aattggtctc
    10801 aggggtaatc tcatatattc tcctgaggct agataaccat ccctccttgt acataatgct
    10861 cagagaaccg tctcttagag gagagatatt ttctatccct cagaaaatcc ccgccgctta
    10921 tccaaccact atgaaagaag gcaacagatc aatcttgtgt tatctccaac atgtgctacg
    10981 ctatgagcga gagataatca cggcgtctcc agagaatgac tggctatgga tcttttcaga
    11041 ctttagaagt gccaaaatga cgtacctatc cctcattact taccagtctc atcttctact
    11101 ccagagggtt gagagaaacc tatctaagag tatgagagat aacctgcgac aattgagttc
    11161 tttgatgagg caggtgctgg gcgggcacgg agaagatacc ttagagtcag acgacaacat
    11221 tcaacgactg ctaaaagact ctttacgaag gacaagatgg gtggatcaag aggtgcgcca
    11281 tgcagctaga accatgactg gagattacag ccccaacaag aaggtgtccc gtaaggtagg
    11341 atgttcagaa tgggtctgct ctgctcaaca ggttgcagtc tctacctcag caaacccggc
    11401 ccctgtctcg gagcttgaca taagggccct ctctaagagg ttccagaacc ctttgatctc
    11461 gggcttgaga gtggttcagt gggcaaccgg tgctcattat aagcttaagc ctattctaga
    11521 tgatctcaat gttttcccat ctctctgcct tgtagttggg gacgggtcag gggggatatc
    11581 aagggcagtc ctcaacatgt ttccagatgc caagcttgtg ttcaacagtc ttttagaggt
    11641 gaatgacctg atggcttccg gaacacatcc actgcctcct tcagcaatca tgaggggagg
    11701 aaatgatatc gtctccagag tgatagatct tgactcaatc tgggaaaaac cgtccgactt
    11761 gagaaacttg gcaacctgga aatacttcca gtcagtccaa aagcaggtca acatgtccta
    11821 tgacctcatt atttgcgatg cagaagttac tgacattgca tctatcaacc ggatcaccct
    11881 gttaatgtcc gattttgcat tgtctataga tggaccactc tatttggtct tcaaaactta
    11941 tgggactatg ctagtaaatc caaactacaa ggctattcaa cacctgtcaa gagcgttccc
    12001 ctcggtcaca gggtttatca cccaagtaac ttcgtctttt tcatctgagc tctacctccg
    12061 attctccaaa cgagggaagt ttttcagaga tgctgagtac ttgacctctt ccacccttcg
    12121 agaaatgagc cttgtgttat tcaattgtag cagccccaag agtgagatgc agagagctcg
    12181 ttccttgaac tatcaggatc ttgtgagagg atttcctgaa gaaatcatat caaatcctta
    12241 caatgagatg atcataactc tgattgacag tgatgtagaa tcttttctag tccacaagat
    12301 ggttgatgat cttgagttac agaggggaac tctgtctaaa gtggctatca ttatagccat
    12361 catgatagtt ttctccaaca gagtcttcaa cgtttccaaa cccctaactg acccctcgtt
    12421 ctatccaccg tctgatccca aaatcctgag gcacttcaac atatgttgca gtactatgat
    12481 gtatctatct actgctttag gtgacgtccc tagcttcgca agacttcacg acctgtataa
    12541 cagacctata acttattact tcagaaagca agtcattcga gggaacgttt atctatcttg
    12601 gagttggtcc aacgacacct cagtgttcaa aagggtagcc tgtaattcta gcctgagtct
    12661 gtcatctcac tggatcaggt tgatttacaa gatagtgaag actaccagac tcgttggcag
    12721 catcaaggat ctatccagag aagtggaaag acaccttcat aggtacaaca ggtggatcac
    12781 cctagaggat atcagatcta gatcatccct actagactac agttgcctgt gaaccggata
    12841 ctcctggaag cctgcccatg ctaagactct tgtgtgatgt atcttgaaaa aaacaagatc
    12901 ctaaatctga acctttggtt gtttgattgt ttttctcatt tttgttgttt atttgttaag
    12961 cgtgggtcgg catggcatct ccacctcctc gcggtccgac ctgggcatcc gaaggaggac
    13021 gcacgtccac tcggatggct aagggagggg cggggatccg gctgctaaca aagcccgaaa
    13081 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
    13141 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat ccactagttc
    13201 tagagcggcc gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg
    13261 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
    13321 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
    13381 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
    13441 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
    13501 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    13561 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    13621 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    13681 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    13741 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    13801 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    13861 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    13921 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    13981 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    14041 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    14101 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
    14161 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    14221 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    14281 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    14341 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    14401 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    14461 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    14521 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    14581 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    14641 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    14701 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    14761 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    14821 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    14881 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    14941 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    15001 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    15061 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    15121 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    15181 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    15241 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    15301 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    15361 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    15421 aagtgccacc tg
//
